Carcinogenesis, Teratogenesis & Mutagenesis ›› 2007, Vol. 19 ›› Issue (2): 125-128.doi: 10.3969/j.issn.1004-616x.2007.02.013

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Injurious Effects of Acridine on Spodoptera Frugiperda 9 Cells and Autographa Californica Multiple Nucleopolyhedrovirus

DENG Ping-jian1,FANG Shi-song1,LI Xi-mei2,NI Hui-bo2,WANG Ye-yuan2,LIN Jian-rong2,   

  1. 1. Shenzhen Disease Control And Prevention Center, Shenzhen 518020; 2. Department of Silk Science, South China Agricultural University, Guangzhou 510642,China
  • Received:2006-01-11 Revised:2006-10-30 Online:2007-03-30 Published:2007-03-30
  • Contact: LIN Jian-rong

Abstract: BACKGROUND & AIM: To study the genetic damage of acridine on insect cells. MATERIALS AND METHODS: Using different concentrations of acridine to treat spodoptera frugiperda 9 cells(sf9),autographa californica multiple nucleopolyhedrovirus(AcMNPV), to study the effect of the acridine on cell growth and development, the percentage of micronucleus formation and the virulence of AcMNPV. RESULTS: The injurious effects of acridine on Sf9 cells in cell activity and micronucleus percentage revealed that cell division and growth became slow,the surface of the cell became rough, and the micronucleus percentage was10.4‰ after treatment with 5 μg/ml acridine. Some cells had broken membranes or died when treated with 10 μg/ml, and with the micronucleus percentage of 22‰, tri-nucleus, multi-nucleus and nuclear cleavage were also found. When acridine-treated AcMNPV infected the sf9 cells, AcMNPV could proliferate within the cell and form polyhedra, but some abnormal polyhedra like triangle or quadrangle were formed at the same time. CONCLUSION: Using certain dosage of acridine to treat sf9 cells and AcMNPV could cause damage to the cell and the formation of abnormal polyhera of AcMNPV.

Key words: acridine, spodoptera frugiperda 9 cell(sf9), autographa californica multiple nucleopolyhedrovirus(AcMNPV), damage

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