Carcinogenesis, Teratogenesis & Mutagenesis ›› 1999, Vol. 11 ›› Issue (3): 113-116.doi: 10.3969/j.issn.1004-616x.1999.03.003

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CLONINGOF GENOMIC FRAGMENT CONTAINING THE EXON 2 OF GLICOCORTICOID RECEPTOR GENE AND CONSTRUCTION OF INDUCIBLE TARGETING VECTOR FOR DELETION OF GR GENE

Yucheng Yao 　Yiping Hu   

  1. Department of Cell Biology , Second Military Medical University , S hanghai 　200433
  • Received:1900-01-01 Revised:1900-01-01 Online:1999-05-30 Published:1999-05-30

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Abstract: To const ruct the inducible targeting vector for the mutagenesis of the mouse glicocorticoid receptor ( GR) gene , we cloned and analyzed a genomic f ragment containing the exon 2 and it s flanking regions of the GR gene , and then , we subcloned some fine regions of the f ragment . Basing on the feature of these regions and the requirement of knockout identification , we const ruct the targeting vector (p Flox2GR) , which provides the important base for the generation of the inducible knockout mice of the GR gene and it s functional analysis.

Key words: Glicocorticoid Receptor Gene, Gene Knockout, Targeting Vector