Abstract: BACKGROUND ＆ AIM： To study the effect of (Haishen polysaccharide peptide,HPP) on the apoptosis and apoptosis gene of mice hepatic cells induced by 60Co-γ irradiation . MATERIAL AND METHODS: Mice were fed 150 mg/kg and 100 mg/kg HPP for four weeks. Mice were then irradiated with 60Co-γ to induce hepatic cells apoptosis once a day five days a week. Four weeks later, in situ cell death detection kit and in situ hybridization were applied to measure qualitative changes at expression level of POD and Caspase-3 positive cells rate to analyze the apoptosis of mice hepatic cells. RESULTS: The rates of POD and Caspase-3 expression in the radiation-injury group were(40.0±3.5)％ and(26.0±1.2)％, respectively,and the rates of the high dose group were(23.9±3.8)％ and (6.0±1.5)％, respectively，the difference between the radiation-injury group and high dose group showed statistical significance (P<0.01).CONCLUSION: HPP had a significant inhibitory effect on the abnormal hepatic expression of POD and Caspase-3 induced by radioactive damage.

Key words: marine biology, in situ hybridization, Caspase-3, apoptosis