Abstract: BACKGROUND ＆ AIM： To establish the techniques of gene chip hybridization, and to verify the reliability of the gene chip data. MATERIAL AND METHODS： Several methods were applied to test the reliability of data, including data normalization treatment, analysis of reference spots, self-comparison test and differential expression experiment. RESULTS： The localized mean normalization method could be used to treat the original data effectively. There was no nonspecific hybridization in the negative control spots, and good reproducibility could be achieved among the repetitive genes in a chip. Meanwhile different batches of chips had good quality and reproducibility, and fluorescein swap labeling had advantages in reducing the staining errors. CONCLUSION： The results proved the validity of the chip hybridization and detection technology established in our lab.

Key words: gene chip, toxicology, analysis of reliability