Carcinogenesis, Teratogenesis & Mutagenesis

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The effects of the different combinations of freezing carrier and sucrose concentration on the oocyte survival after thawing

ZHANG Hong-qin,LIU Si-yao,HUANG Jun,MENG Xiang-qian,AI Ling,LEI Wan-qiu,ZHONG Ying*   

  1. Center for Reproductive Medicine, Chengdu Jinjiang Hospital for Maternal and Child Health Care, Chengdu 610016, Sichuan, China
  • Received:2012-08-30 Revised:2012-09-21 Online:2011-11-30 Published:2011-11-30
  • Contact: ZHONG Ying,E-mail:yzhong08@126.com
  • About author:张红琴 (1980- ),女,山西人,硕士,研究方向:胚胎工程。
  • Supported by:

    四川省卫生厅科学研究基金项目 (090099)

Abstract:

OBJECTIVE: To investigate the effects of different combinations of sucrose concentration and freezing carrier on oocyte survival after thawing. METHODS:853 failed-fertilization oocytes after conventional IVF were collected and frozen with vitrification method in Center for Reproductive Medicine,Chengdu Jinjiang Hospital for Women and Child Health Care from June 2009 to May 2012. All oocytes were divided into four groups;group A:79 oocytes were vitrified with the cryoloop carrier and thawed using the conventional method;group B:580 oocytes were vitrified with the hemi-straw carrier and thawed using the conventional method;group C:121 oocytes were vitrified with the cryoloop carrier and thawed using the modified method;group D:73 oocytes were vitrified with the hemi-straw carrier and thawed using the modified method. After thawing for 2 h,the oocyte survival rates were compared among the groups mentioned above. RESULTS:The rate of oocyte survival in group C (97.52%) was significantly higher than those in group A (72.15%),group B (52.24%) and group D (64.38%) (P< 0.01,respectively). The rate of oocyte survival in group A was significantly higher than that in group B (P< 0.05). No statistical difference in the rate of oocyte survival was found between other groups (P>0.05). CONCLUSION:The survival rate of MⅡ oocytes could be improved when oocytes were vitrified with the cryoloop carrier and thawed using the modified method.

Key words: sucrose concentration, frozen carrier, oocytes, vitrification