Carcinogenesis, Teratogenesis & Mutagenesis

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Inhibitory action of the daidzein on organochlorine pesticide- induced MCF-7 breast cancer cell multiplication

ZHANG Ming,NA Xiao-lin*,YANG Yang,YI Cheng   

  1. Department of Environmental Health, College of Public Health, Harbin Medical University, Harbin 150081, Heilongjiang, China
  • Received:2012-09-18 Revised:2012-11-29 Online:2013-03-30 Published:2013-03-30
  • Contact: NA Xiao-lin,E-mail:naxiaolin1495@sohu.com;Tel: 0451-89502902

Abstract:

 OBJECTIVE: To investigate the combined action of inhibitory daidzein and organochlorine pesticides on MCF-7 cell multiplication. METHODSMCF-7 human breast carcinoma cells in logarithmic growth phase were exposed to each of daidzein,chlordane,β-HCH,o,p'-DDT in different concentration alone and another group with 100 μmol/L daidzein combined with each pesticide. A solvent control group (0.1% DMSO) was set up. After 48 h of incubation,the cell multiplication was assessed by MTT. RESULTS:Compared with solvent group,the 100 μmol/L daidzein only group reduced cell multiplication. All different concentrations of each organochlorine pesticide could increase cell multiplication (P<0.05). Compared with the single agent treatment group,the rate of cell multiplication in combined treatment group was significantly reduced (P<0.05). CONCLUSION:Daidzein could inhibit cell multiplication induced by different concentrations of organochlorine pesticides.

Key words: daidzein, organochlorine pesticide, cell multiplication, breast cancer cells