Abstract:

 OBJECTIVE: To provide the basis for studies on the biological dose estimation of peripheral blood lymphocyte in molecular level and the mechanism of radiation injury. METHODS：Using whole genome microarray technology，the gene expression profiles and the pathways of the rat peripheral blood lymphocytes were studied 6，12，24 h after irradiated by 2.0 Gy γ ray. The results were verified by fluorescence quantitative PCR technology. RESULTS：At 6 h，there were 534 differentially expressed genes，of which 332 genes were up-regulated and 202 genes down-regulated. At 12 h，there were 2 001 differentially expressed genes，of which 1 258 were up-regulated and 743 down-regulated. At 24 h，there were 6 925 differentially expressed genes，of which 2 814 were up-regulated and 4 111 down-regulated. There were 270 common differentially expressed genes at three time points，of which 143 were up-regulated and 127 genes down-regulated. In the analysis of differentially expressed genes，27 pathways were related to the differentially expressed genes at 6 h，22 pathways related at 12 h，and 41 pathways related at 24 h. The relative quantitative results of RT-PCR were consistent with the results of gene chip test in Trmt61a，Tlr3 and Enc1 genes. CONCLUSION：The differentially expressed genes of rat peripheral blood lymphocytes increased time after irradiation，and the screened pathways were also different. The mechanisms involved in radiation injury were also different  at various time poins after irradiation.

Key words: &gamma, ray, rat, lymphocyte, gene chip, differential expression gene