Carcinogenesis, Teratogenesis & Mutagenesis ›› 2001, Vol. 13 ›› Issue (2): 77-080.doi: 10.3969/j.issn.1004-616x.2001.02.004

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INFLUENCE ON THE TUMORIGENICITY OF THE HUMAN RENAL CELL CARCINOMA ( RCC) CELLS TRANSDUCED WITH HUMAN TNFα GENE

ZHANG Xiao-qin1, CHEN Jian2, CAO Guang-wen3, WAN Mo-bing1, QI Zhong-tian3.   

  1. 1. Infection Department, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2. Department of Military Hygiene, Second Military Medical University, Shanghai 200433, China; 3. Department of Microbiology, Second Military Medical University, Shanghai 200433, China
  • Received:2000-08-29 Revised:2000-10-31 Online:2001-04-30 Published:2001-04-30

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Abstract: Purpose : To study the influence on the tumorigenicity of the human renal cell carcinoma (RCC) cells by t ransfection of human TNFαgene. Methods : hTNFαcDNA was inserted into ret roviral vector to const ruct recombinant vector pL ( TNFα) SN. The packaged and confirmed ret rovirus were infected to RCC cell line.Then , nude mice were injected by 78620 cell infected with the hTNFαgene or not , and the tumorigenecity of RCC was observed. Results : The mean expression level of TNFαin the supernatant of TNFα2t ransfected 78620 cell2culture medium was (5 004 ±624) pg/ ml. And the nude mice injected with TNFα gene engineered RCC cells had no tumor growth. Conclusion : The TNFαgene engineered human RCC cells lost tumorigenicity.

Key words: TNFα, renal cell carcinoma, tumorigenicity

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