卡铂联合TRAIL对肺癌A549细胞增殖和凋亡的影响

doi:10.3969/j.issn.1004-616x.2015.02.007

Abstract

Abstract:

OBJECTIVE:To study the effects of carboplatin combined with TRAIL (TNF- related apoptosis inducing ligand) on proliferation and apoptosis of human lung cancer A549 cells. METHODS:After treated by TRAIL, carboplatin or combination of two drugs, the proliferation of A549 was measured by MTS and apoptosis rate was evaluated by flow cytometry. The morphology was investigated by microscopy. RT-PCR and Western blot were used to examine the gene and protein expressions of DR4, DR5, Survivin and XIAP. RESULTS:Carboplatin or TRAIL alone or combined inhibited the proliferation of A549 cells in concentration-dependent (20-80 μg/mL) manner. Carboplatin combined with TRAIL exhibited a stronger inhibitory effect and induced apoptosis than when used alone. Carboplatin could down-regulate the protein expression level of Survivin and XIAP and increase the DR5 protein expression level, but have no effect on DR4 expression. CONCLUSION:Combination of carboplatin and TRAIL could inhibit proliferation and apoptosis via influencing the expressions of Survivin, XIAP and DR5 in A549 cells.

Key words: carboplatin, TRAIL, A549, proliferation, apoptosis

CLC Number:

R730.2

WANG Huimin, WANG Hongying, DAI Suli, LI Lei, ZHAO Lianmei. Effects of carboplatin combination with TRAIL on the proliferation and apoptosis of lung adenocarcinoma cancer cell line A549[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(2): 111-115.

References

[1] 陈万青, 张思维, 邹小农. 中国肺癌发病死亡的估计和流行趋势研究[J]. 中国肺癌杂志, 2010, 13 (5):488-493.
[2] Sandler A, Gray R, Perry MC, et al. Paclitaxel-carboplatin alone or with bevacizumab for non-small-cell lung cancer[J]. N Engl J Med, 2006, 355(24):2542-2550.
[3] Takayama K, Inoue K, Tokunaga S, et al. Phase II study of concurrent thoracic radiotherapy in combination with weekly paclitaxel plus carboplatin in locally advanced non-small cell lung cancer:LOGIK0401[J]. Cancer Chemoth Pharm, 2013, 72(6):1353-1359.
[4] Schneider PD, Olson A, Tardivel B, et al. Identification of a new murine tumor necrosis factor receptor locus that contains two novel murine receptors for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)[J]. J Biol Chem, 2003, 278(7):5444-5454.
[5] Matsuzaki H, Schmied BM, Ulrich A, et al. Combination of tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) and actinomycin D induces apoptosis even in TRAIL- resistant human pancreatic cancer cells[J]. Clin Cancer Res, 2001, 7(2):407-414.
[6] Hwang H, Biswas R, Chung PS, et al. Modulation of EGFR and ROS induced cytochrome c release by combination of photodynamic therapy and carboplatin in human cultured head and neck cancer cells and tumor xenograft in nude mice[J]. J Photoch Photobiol B, 2013, 128(1):70-77.
[7] Mahalingam D, Szegezdi E, Keane M, et al. TRAIL receptor signalling and modulation:Are we on the right TRAIL[J]. Cancer Treat Rev, 2009, 35(3):280-288.
[8] Maduro JH, Noordhuis MG, ten Hoor KA, et al. The prognostic value of TRAIL and its death receptors in cervical cancer[J]. Int J Radiat Oncol Biol Phys, 2009, 75(1):203-211.
[9] 孙非凡, 孙铭娟, 王梁华, 等. TRAIL联合吉西他滨对肺癌NCI-H460细胞增殖和凋亡的影响[J]. 中国肿瘤生物治疗杂志, 2011, l (3):296-300.
[10] Zhai G, Kim H, Sarver D, et al. Early therapy assessment of combined anti-DR5 antibody and carboplatin in triple-negative breast cancer xenografts in mice using diffusion-weighted imaging and 1H MR spectroscopy[J]. J Magn Reson Imaging, 2014, 39(6):1588-1594.
[11] Deveraux QL, Takahashi R, Salvesen GS, et al. X-linked IAP is a direct inhibitor of cell-death proteases[J]. Nature, 1997, 388 (6639):300-304.
[12] Truneh A, Sharma S, Silverman C, et al. Temperature-sensitive differential affinity of TRAIL for its receptors DR5 is the highest affinity receptor[J]. J Biol Chem, 2000, 275(30): 23319 -23325.

[1]	ZHAO Yan, SUN Zhenxiao. Metformin inhibitd proliferation and migration of human breast cancer cell in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 35-39.
[2]	WANG Xiaozhou, LI Hongshi, YU Xi, WEI Ning, WANG Ziying, HE Lijie. Effects of sinomenine on proliferation and invasion of HPV-positive cervical cancer SiHa cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(6): 442-445.
[3]	LI Boru, QIN Shuangjian, GUAN Lan, LI Runbing, CAI Ying, PU Jiening, ZENG Ming, XIAO Fang, XU Xinyun. Effects of PM_2.5 on oxidative damage and apoptosis in HK-2 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 360-364.
[4]	HUANG Xiaohua, NIU Yongdong, SHI Ganggang. Inhibitory effects and mechanisms of nuclear receptor FXR activation in cervical cancer cell line CaSki [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 302-306.
[5]	WANG Xikai, MENG Qinghe, GAO Yanlu. miR-223-3p negatively regulated ECT2 to induce apoptosis in non-small cell lung cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 187-192.
[6]	ZHENG Junwen, CHANG Chen, HAO Jiajie, CAI Yan, WANG Mingrong, ZHANG Yu. Investigation on expression and function of NEDD4 in esophageal squamous cell carcinoma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 203-207,224.
[7]	ZHANG Na, FAN Zhilu, YANG Liyan, CHANG Chen, CAI Yan, HAO Jiajie, WANG Mingrong. Clinical significance of decreased SERPINB5 expression in esophageal squamous cell carcinomas [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 101-109.
[8]	LI Boru, QIN Shuangjian, LI Runbing, CAI Ying, ZHENG Kai, ZENG Ming, XIAO Fang, XU Xinyun. Effects of p38MAPK gene on PM_2.5-induced expression of oncogenes and apoptosis genes in HK-2 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 129-135,142.
[9]	FANG Lei, YANG Tao, SHENG Lei, MUTALIFU·Aimaiti, QI Xinxin, AINIWAER·Aikemu, YILIYAER·Nijiati. Biological effects of saponins from the seeds of Nigella glandulifera Freyn on human cervical cancer SiHa cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 143-148,152.
[10]	MA Shunpeng, WANG Quankai, WANG Miao, SONG Jiayang, WUHAN Baolier, XIE Guangyun, XU Jianning. Long non-coding RNA regulates apoptosis during glycidyl methacrylate-induced malignant transformation of 16HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 1-5,31.
[11]	LI Boru, QIN Shuangjian, LI Runbing, CAI Ying, ZHENG Kai, WANG Bingyu, ZENG Ming, XIAO Fang, XU Xinyun. Effects of PM_2.5 exposure on expression of oncogenes and apoptosis genes in human renal epithelial cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 6-11.
[12]	HUANG Lili, ZHANG Mengyun, LIU Keliang, PANG Dingguo, LIU Lida, XU Peiyu. Hepatotoxicity of 2,4-dichlorophenoxyacetic acid in young SD rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 22-27.
[13]	ZHAO Junwei, HUA Chenfeng, SHANG Pingping, XIE Fuwei, LI Xiang. Induction of oxidative stress by arsenious acid As(Ⅲ) in A549 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 28-31.
[14]	CUI Zhaoyang, LI Suna, JIANG Xuqian, WANG Yidan, HOU Liying. Activation of the TRAIL death receptor by apigenin for induction of apoptosis in the gastric cancer SGC-7901 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 32-36.
[15]	LAN Li, CHEN Haili, XU Lei. Mechanism of RBMS3-S3 on the proliferation, apoptosis and invasion of cervical cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 438-443.

Effects of carboplatin combination with TRAIL on the proliferation and apoptosis of lung adenocarcinoma cancer cell line A549

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments