利用胚胎干细胞试验模型评价矮壮素的发育毒性

doi:10.3969/j.issn.1004-616x.2015.04.002

Abstract

Abstract: OBJECTIVE: To investigate the developmental toxicity and underlying mechanism of chlormequat chloride by utilizing embryonic stem cell test in vitro. METHODS: Mouse embryonic stem cells and 3T3 cells were treated with different doses of chlormequat chloride,the effects on ES and 3T3 cell proliferation and ES cell differentiation were determined. A cascade of genes related to normal cardiac development was examined by real time PCR. RESULTS: Chlormequat chloride (1 000 μg/mL) could evidently increase the viability of mouse fibroblasts 3T3 cells and mouse embryonic stem cells. Chlormequat chloride could influence embryonic stem cell cardiac differentiation in a U shape manner. Compared to the control group,only 69.4% beating EBs was observed at 250 μg/mL concentration. Chlormequat chloride was able to cause effect on gene expression during cardiogenesis,especially Nkx2.5 and α-MHC. CONCLUSION: Chlormequat chloride could not be classified according to the prediction model recommended by ECVAM. Chlormequat chloride at 250 μg/mL concentration could retard embryonic stem cell differentiation into myocardial cell without causing any morphological malformations of 3T3 and ES-D3. Chlormequat chloride could alter the expression of crucial genes during the early stages of cardiogenesis,and these effects might result in adverse developmental outcomes.

Key words: chlormequat chloride, plant growth regulators, developmental toxicity, embryonic stem cell test model

CLC Number:

R394.6

KONG Dan, HAO Weidong, JIANG Jianjun, SHANG Lanqin, WEI Xuetao, SHI Fuli. Developmental toxicity assessment of chlormequat chloride using embryonic stem cell test[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(4): 260-265,271.

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Developmental toxicity assessment of chlormequat chloride using embryonic stem cell test

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