Pokemon基因干扰对泌乳素腺瘤细胞的影响及其分子机制

doi:10.3969/j.issn.1004-616x.2016.03.006

Abstract

Abstract: OBJECTIVE:Prolactinoma is a common intracranial tumor wihch always leads to serious clinical outcomes. This study aims to confirm the association between the proto-oncogene pokemon and prolactinoma progression, and further explore the molecular mechanisms. METHODS:Tumor tissues were collected from patients with prolactinoma. The primary prolactinoma cells were digested from the tissues and cultured with proper conditions. The expression of pokemon gene was knocked-down by siRNA transfection, and the proliferation of prolactinoma primary cells was analyzed by CCK8 assay. The expression level of pokemon related genes, cyclin-A, p14ARF and p21 in tissues and prolactinoma primary cells were examined by Western blot analysis. RESULTS:Expression of pokemon in prolactinoma primary cell was successfully knocked-down by siRNA transfection. The results showed that siRNA2 showed the highest efficiency of inhibiting the proliferation ability of prolactinoma primary cells. Cyclin-A, p14ARF and p21 were down-regulated by pokemon in both prolactinoma tissues and primary cells. CONCLUSION:Pokemon could improve prolactinoma progression by reducing the expression level of tumor inhibition factors, cyclin-A, p14ARF and p21.

Key words: prolactinoma, primary culture, pokemon, cell progression, cyclin-A, p14ARF, p21

CLC Number:

R736.4

YU Haizhou, TANG Aifa, LI Weiping, LIU Yufei, YUAN Jianhui. Pokemon gene induced prolactinoma progression and related molecular mechanisms[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 190-194.

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Pokemon gene induced prolactinoma progression and related molecular mechanisms

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