Abstract: Purpose: To provide toxicity data of Stamen Nelumbinis(SN) for clinic application. Methods:Acute toxicity: groups of mice for four doses(21.5, 10.0, 4.64, 2.15 g / kg) were administrated by gavage. Ames test: groups for five doses(0.5, 5, 50, 500, 5 000 μg / plate) were divided to observe the effects of SN on strains of TA 97, TA 98, TA 100, TA 102 with or without S 9 mixture added. Micronucleus test of bone marrow PCE cell in mice: Three groups for 2.5, 5.0, 10.0 g / kg were divided. After the animals received SN by gavage two times, they were killed. Then the slides were made and observed. Sperm shape abnormality test in mice: three groups for 0.5, 5.0, 10.0 g / kg were divided. The animals were killed on 35 d after they received SN by gavage for 5 d. The slides were made and observed. Results: SN was a substance with no toxicity according to the acute toxicity. Ames test: the result of SN was negative. SN had no mutagenesis on bone marrow PCE cells in mice, nor mutagenesis on sperm shape. Conclusion: The toxicity of SN was low and has not genetic toxicity.

Key words: stamen nelumbinis, genetic toxicity, acute toxicity