新疆黑种草子总黄酮体外抗宫颈癌活性研究

doi:10.3969/j.issn.1004-616x.2019.05.009

Abstract

Abstract: OBJECTIVE:The aim of this study was to investigate the toxicological activityity of total flavonoids from Xinjiang semen Nigellae,the unique national drug resources of Xinjiang,in human cervical cancer cell lines to investigate the mechanism by using. METHODS:Two humna cervical carcinoma cell lines,SiHa and HeLa,were treated with different concentrations of the total flavonoids from the semen nigellae(2.5,5,10,15,20,25,50 μg/mL). Their toxicological activities were detected using the MTT assay which determined the effective concentrations and IC₅₀. Anti-migration effects were detected by cell scratch assay. Apoptosis rates were detected by flow cytometry. RESULTS:Compared with the control group,proliferation of the treated cervical cancer cells was inhibited in a concentration-dependent manner. The IC₅₀ of total flavonoids was 16.935 μg/mL for SiHa and 18.366 μg/mL for HeLa. By comparing the scratch widths before and after the treatments,the relative migration distance of tumor cells in the SiHa cell line control group was (223.333±13.868) px,and that in the treated group was (56.333±10.970) px. The relative migration distance of the HeLa cell control group was (360.667±15.308) px,and that in the treated group was (13.000±3.606) px. Total flavonoids also induced apoptosis in both cervical cancer cell lines. The apoptosis rates in the 10,15 and 25 μg/mL treatment groups were (8.5±0.5)%,(35.9±1.3)% and (85.7±1.6)% for SiHa,and were (5.3±0.4)%,(11.4±0.8)% and (85.9±1.9)% for HeLa. The differences were significantly different from that of the control (P < 0.05). CONCLUSION:The total flavonoids from Xinjiang semen nigellae demonstrated anti-proliferation anti-migration activities in vitro and induced apoptosis in human cervical cancer cell lines.

Key words: semen Nigellae, cervical cancer, proliferation, migration, apoptosis

CLC Number:

R737.33

SHENG Lei, WU Guizhen, REYIMU Renaguli, AIKEMU Ainiwaer. Toxicological activities of total flavonoids from Xinjiang semen Nigellae in human cervical cancer cell lines[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 385-391.

References

[1] 艾尼娃尔·艾克木, 美丽万·阿不都热依木, 王岩, 等. 维吾尔药黑种草子中总黄酮含量的测定[J]. 时珍国医国药, 2008, 19(5):1167-1168.
[2] 信学雷, 薛慧清, 阿吉艾克拜尔·艾萨, 等. 黑种草属植物研究进展[J]. 时珍国医国药, 2008, 19(6):1514-1517.
[3] 张来, 杨碧昌, 刘宁. 植物黄酮类化合物在医药上的应用[J]. 安顺学院学报, 2010, 12(2):84-88.
[4] 苗金玲, 王文锋, 潘景喜, 等. CCl₃OO·槲皮素、芦丁、EGCG的分子反应机理[J]. 中国科学:B辑化学, 2001, 31(4):339-343.
[5] 黎丹戎, 侯华新, 张玮, 等. 汉黄芩素诱导人卵巢癌细胞A2780凋亡及对细胞端粒酶活性的影响[J]. 癌症:英文版, 2003, 22(8):801-805.
[6] 侯华新, 黎丹戎, 秦箐, 等. 黄芩黄酮A对人肝癌细胞7402的抑制能力及体外增效作用[J]. 中国临床药学杂志, 2000, 9(3):166-168.
[7] WANG I K, LIN-SHIAU S Y, LIN J K. Induction of apoptosis by apigenin and related flavonoids through cytochrome c release and activation of caspase-9 and caspase-3 in leukaemia HL-60 cells[J]. Eur J Cancer, 1999, 35(10):1517-1525.
[8] 王怡君, 南存金, 陈映鹤. 芹菜素对激素非依赖性前列腺癌细胞株PC-3的抑制作用[C]//2015年浙江省泌尿外科学男科学学术年会论文集. 浙江湖州, 2015:161.
[9] FUNG K P, WU T W, LUI C P. Purpurogallin inhibits DNA synthesis of murine fibrosarcoma L-929 and human U-87 MG glioblastoma cells in vitro[J]. Chemotherapy, 1996, 42(3):199-205.
[10] YANG B H, BRAY F I, PARKIN D M, et al. Cervical cancer as a priority for prevention in different world regions:an evaluation using years of life lost[J]. Int J Cancer, 2004, 109(3):418-424.
[11] COHEN J. Public health. High hopes and dilemmas for a cervical cancer vaccine[J]. Science, 2005, 308(5722):618-621.
[12] 国家癌症中心.2017中国肿瘤登记年报[M].北京:人民卫生出版社, 2018:141-145.
[13] 拉莱·苏祖克, 彭玉华, 周康, 等. 新疆不同民族子宫颈癌发病趋势分析[J]. 新疆医科大学学报, 2006, 29(7):569-571.
[14] 韩英, 孟炜, 陈志芳, 等. 适宜新疆南部地区少数民族妇女宫颈癌筛查方法的研究[J]. 现代妇产科进展, 2008, 17(2):132-133.
[15] 尹燕娜, 艾星子·艾里, 丁岩. 新疆维吾尔族宫颈癌发病特征及进展[J]. 现代妇产科进展, 2012, 21(5):404-405.
[16] 刘伟平, 张永俐, 孙加林. 铂类抗癌药物展望[J]. 贵金属, 2005, 26(1):47-52.
[17] 贺清波, 闫涛. 术前放疗与术前化疗治疗Ⅰb2期宫颈癌的疗效比较[J]. 实用癌症杂志, 2015, 30(12):1906-1908.
[18] 曾筱怡. 不同化疗方案联合同步放疗治疗中晚期宫颈癌的疗效分析[J]. 江西医药, 2015, 50(10):1063-1065.
[19] 唐滟, 袁亚维. 宫颈癌调强放射治疗同步化学药物治疗疗效及毒副反应分析[J]. 中国现代医学杂志, 2015, 25(36):68-72.
[20] 张芸, 龚建忠, 林小梅. 同步放化疗在宫颈癌治疗中的疗效及安全性观察[J]. 中国实用医药, 2015, 10(33):12-13.
[21] 李娜, 高俊岩, 刘敏. 细胞凋亡和肿瘤的关系研究进展[J]. 当代医学, 2009, 15(16):13-14.
[22] HICKMAN J A. Apoptosis induced by anticancer drugs[J].Cancer Metastasis Rev, 1992, 11(2):121-139.

Toxicological activities of total flavonoids from Xinjiang semen Nigellae in human cervical cancer cell lines

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHAO Yan, SUN Zhenxiao. Metformin inhibitd proliferation and migration of human breast cancer cell in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 35-39.
[2]	WANG Xiaozhou, LI Hongshi, YU Xi, WEI Ning, WANG Ziying, HE Lijie. Effects of sinomenine on proliferation and invasion of HPV-positive cervical cancer SiHa cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(6): 442-445.
[3]	YANG Xingxiao, ZHANG Xueyuan, ZOU Naiyi, SHAN Bao'en, MA Ming, ZHU Shuchai. Effects of HMGB1 silencing on migration, invasion and radiosensitivity of esophageal squamous cell carcinoma cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 327-333.
[4]	LI Boru, QIN Shuangjian, GUAN Lan, LI Runbing, CAI Ying, PU Jiening, ZENG Ming, XIAO Fang, XU Xinyun. Effects of PM_2.5 on oxidative damage and apoptosis in HK-2 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 360-364.
[5]	HUANG Xiaohua, NIU Yongdong, SHI Ganggang. Inhibitory effects and mechanisms of nuclear receptor FXR activation in cervical cancer cell line CaSki [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 302-306.
[6]	WANG Xikai, MENG Qinghe, GAO Yanlu. miR-223-3p negatively regulated ECT2 to induce apoptosis in non-small cell lung cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 187-192.
[7]	ZHENG Junwen, CHANG Chen, HAO Jiajie, CAI Yan, WANG Mingrong, ZHANG Yu. Investigation on expression and function of NEDD4 in esophageal squamous cell carcinoma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 203-207,224.
[8]	ZHANG Na, FAN Zhilu, YANG Liyan, CHANG Chen, CAI Yan, HAO Jiajie, WANG Mingrong. Clinical significance of decreased SERPINB5 expression in esophageal squamous cell carcinomas [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 101-109.
[9]	LI Boru, QIN Shuangjian, LI Runbing, CAI Ying, ZHENG Kai, ZENG Ming, XIAO Fang, XU Xinyun. Effects of p38MAPK gene on PM_2.5-induced expression of oncogenes and apoptosis genes in HK-2 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 129-135,142.
[10]	FANG Lei, YANG Tao, SHENG Lei, MUTALIFU·Aimaiti, QI Xinxin, AINIWAER·Aikemu, YILIYAER·Nijiati. Biological effects of saponins from the seeds of Nigella glandulifera Freyn on human cervical cancer SiHa cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 143-148,152.
[11]	MA Shunpeng, WANG Quankai, WANG Miao, SONG Jiayang, WUHAN Baolier, XIE Guangyun, XU Jianning. Long non-coding RNA regulates apoptosis during glycidyl methacrylate-induced malignant transformation of 16HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 1-5,31.
[12]	LI Boru, QIN Shuangjian, LI Runbing, CAI Ying, ZHENG Kai, WANG Bingyu, ZENG Ming, XIAO Fang, XU Xinyun. Effects of PM_2.5 exposure on expression of oncogenes and apoptosis genes in human renal epithelial cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 6-11.
[13]	HUANG Lili, ZHANG Mengyun, LIU Keliang, PANG Dingguo, LIU Lida, XU Peiyu. Hepatotoxicity of 2,4-dichlorophenoxyacetic acid in young SD rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 22-27.
[14]	CUI Zhaoyang, LI Suna, JIANG Xuqian, WANG Yidan, HOU Liying. Activation of the TRAIL death receptor by apigenin for induction of apoptosis in the gastric cancer SGC-7901 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 32-36.
[15]	LAN Li, CHEN Haili, XU Lei. Mechanism of RBMS3-S3 on the proliferation, apoptosis and invasion of cervical cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 438-443.