齐墩果酸对酒精诱导的大鼠胃壁氧化损伤的保护作用

doi:10.3969/j.issn.1004-616x.2021.05.009

Abstract

Abstract: OBJECTIVE: To study the protective effect of oleanolic acid (OA) on alcohol-induced gastric mucosal injury in rats. METHODS: Using the random number table method, 24 SD rats were randomly divided into 3 groups of 8 rats per group:control (isocaloric glucose solution),alcohol exposure (gavage 4 g/kg alcohol to construct alcoholic gastric wall injury model),OA intervention (perfusion to the stomach with an alcohol solution of 10 mg/kg OA). All treatments lasted 30 days. Rats were evaluated for general changes of the stomach wall and pathological changes of HE; content of malondialdehyde (MDA) and oxidized glutathione (GSSG) in the stomach wall; content of reduced glutathione (GSH) and ratio of GSH/GSSG,antioxidant enzyme transcription Factor Nrf-2 mRNA and protein expression; and TNF-α, IL-6 mRNA and protein expression levels. RESULTS: Compared with the control group,4 g/kg alcohol exposure for 30 days successfully induced oxidative damage and inflammatory reactions of the gastric wall in rats. Compared with the alcohol-exposed rats, pathological damage to the stomach wall in the OA intervention group was reduced, contents of MDA and GSSG in the stomach wall tissue were decreased (P < 0.05),GSH contents and the GSH/GSSG ratios were increased (P < 0.05),and Nrf-2 was increased. mRNA and protein expression levels were increased significantly (P< 0.05),while mRNA and protein expressions of TNF-α and IL-6 were decreased (P < 0.05). CONCLUSION: OA played a protective role on alcohol-induced gastric wall injury in rats through its antioxidant and antiinflammatory effects.

Key words: oleanolic acid, alcohol, oxidative stress, inflammation

CLC Number:

R994.6

LI Hongwei, KONG Deqin, YU Weihua, WU Hao, WANG Zhao, LIU Rui, HAI Chunxu, WANG Xin, LIU Jiangzheng, LI Wenli. Protective effect of oleanolic acid co-treatment on alcohol-induced oxidative damage in gastric mucosa of rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 365-369.

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Protective effect of oleanolic acid co-treatment on alcohol-induced oxidative damage in gastric mucosa of rats

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