Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (5): 329-333.doi: 10.3969/j.issn.1004-616x.2009.05.001

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Effect of NADH on Mutations of p53 Gene and Expression of c-erbB2 Gene in L02 Cells

WEN Ju-yi1; ZHANG Ji-ren2; LI Peng2; ZHANG Jun1; WANG Bin1   

  1. 1.Department of Radiotherapy and Oncology, The Navy General Hospital, Beijing 100037;2.Center of Oncology, Zhujiang Hospital of Southern Medical University, Guangzhou 510280,China
  • Received:2009-02-11 Revised:2009-03-12 Online:2009-09-30 Published:2009-09-30
  • Contact: WEN Ju-yi

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Abstract: BACKGROUND AND AIM: To investigate effects of NADH on mutations of p53 gene and c-erbB2 gene in L02 cells induced by diethylnitrosamine(DENA). MATERIALS AND METHODS: The anti-mutagenesis effects of NADH on DENA-induced mutations of p53 gene in L02 cells were investigated by PCR-SSCP and PCR-RFLP analysis. The effect of NADH on c-erbB2 gene amplification was assessed by Southern blotting analysis. RESULTS: PCR-SSCP analysis showed that the mutation rate of p53 exon7 in DL02-Ⅲ and DL02-B cells in NADH protection groups decreased to 33.3％(4/12)and 50％(6/12), respectively. The difference was significant(P<0.01)compared with DENA mutagenesis group. PCR-RFLP analysis showed that NADH decreased the mutation frequency of the 249th codon of p53 in L02 cells induced by DENA. Southern blotting showed that NADH down-regulated the amplification of c-erbB2 gene in L02 cells induced by DENA. CONCLUSION: Reduced coenzyme NADH had anti-mutagenesis effects and could decrease mutation of p53 and inhibit the expression of c-erbB2 in L02 cells induced by DENA.

Key words: nicotinamide adenine dinucleotide-reduced(NADH), anti-mutagenesis, diethylnitrosamine

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