COL5A2蛋白表达与胃癌患者临床病理指标和预后的关系

doi:10.3969/j.issn.1004-616x.2025.03.011

Abstract

Abstract: OBJECTIVE： To analyze correlations between COL5A2 expression and clinical pathological characteristics of gastric cancer patients. METHODS： Among gastric cancer patients，146 of them with radical surgery were recruited. Their tumor and adjacent tissues were taken for immunohistochemical staining to detect the expression of COL5A2 protein and analyze its relationship with clinical pathological indicators，including prognosis of patients. Gastric cancer cell lines (MKN-45 and AGS) were infected with lentivirus carrying shRNA (short hairpin RNA) vector and COL5A2 gene vector to obtain gastric cancer cell strains with stable knockdown and overexpression of COL5A2， respectively. The effect of knocking down and overexpressing COL5A2 on the proliferation of MKN-45 and AGS gastric cancer cells was observed. RESULTS： The positive expression rate of COL5A2 protein in tumor tissues was 39.7% ， which was higher than that in adjacent tissues. The proportions of T3-T4 stage (58.6%)，N1-N3 stage (63.8%)，and stage III (56.9%) in the COL5A2- positive tumor tissues were higher than that in the COL5A2-negative tissues (36.4% ， 36.4% and 29.5% ， respectively，all P<0.05). Survival analysis showed that the recurrence-free survival rate and overall survival rate of patients in the COL5A2-positive group were lower than those in the COL5A2-negative group (P<0.05). Gastric cancer cell lines overexpressing COL5A2 showed significantly enhanced cell proliferation. Knocking down COL5A2 significantly inhibited the proliferation of gastric cancer cells. CONCLUSION： COL5A2 was highly expressed in tumor tissues of gastric cancer patients，indicating later TNM staging and poorer patient prognosis. Overexpression of COL5A2 promoted gastric cancer cell proliferation in vitro. COL5A2 may be useful as a new biomarker for predicting the prognosis of gastric cancer patients.

Key words: gastric cancer, COL5A2, clinical prognosis, clinical pathological characteristics, cell proliferation

CLC Number:

R735.2

HOU Huiyun, BAI Feihu, YAN Xi, WU Lihong, ZUO Pengxi, WANG Jun. Correlations between COL5A2 protein expression and clinicopathological parameters in patients with gastric cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2025, 37(3): 242-247.

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Correlations between COL5A2 protein expression and clinicopathological parameters in patients with gastric cancer

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