敲低B7-H4对MFC细胞增殖和肿瘤形成的影响及其机制

doi:10.3969/j.issn.1004-616x.2026.01.001

Abstract

Abstract: OBJECTIVE： To investigate effects of the co-stimulatory molecule B7-H4 on MFC cell proliferation and tumor formation， and to explore its potential mechanisms. METHODS： MFC cells were transfected with lentiviruses to knock down B7-H4 expression，and stable transfection cell lines were screened. The cells were divided into a knockdown group (transfected with B7-H4 knockdown lentivirus) and a control group (transfected with the empty vector lentivirus). Expression levels of B7-H4 mRNA and protein in MFC cells were detected using RT-qPCR and Western blot to evaluate knockdown effects. Impact of B7-H4 knockdown on MFC cell proliferation and migration was assessed through CCK-8 assay and cell scratch experiments. The stably transfected cells from both groups were then injected subcutaneously into mice， and tumor formation was evaluated through tumor transplantation experiments. Expression levels of B7-H4，WNT7A，and STAT3 in MFC cells and mouse tumor tissues were analyzed using RT-qPCR and Western blot. RESULTS： RT-qPCR and Western blot results showed that compared to the control group，expression levels of B7-H4 in the knockdown group were significantly reduced (P<0.01)，indicating successful construction of B7-H4 knockdown cell lines. In MFC cells， B7-H4 knockdown significantly reduced both proliferation and migration capacity compared to the control group (P<0.01). In subcutaneous tumor formation experiments， the tumor volume and mass in the knockdown group were significantly smaller than those in the control group (P<0.01). RT-qPCR and Western blot results demonstrated that expression levels of B7-H4， WNT7A， and p-STAT3 in the knockdown group were significantly lower than those in the control group (P<0.01). In contrast， total STAT3 expression showed no significant change (P>0.05). CONCLUSION： B7-H4 knockdown inhibited MFC cell proliferation and migration and affected tumor formation in MFC cells. Low expression of B7-H4 inhibited expression of WNT7A and STAT3.

Key words: B7-H4, cell proliferation, cell migration, tumor formation, WNT7A

CLC Number:

R735.2

GUO Ya, GUO Teng, LIU Mingfeng, DU Liying, WEN Tianjiao, CHEN Xinran. The effect of B7-H4 knockdown on MFC cell proliferation and tumor formation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2026, 38(1): 1-6.

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The effect of B7-H4 knockdown on MFC cell proliferation and tumor formation

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