Abstract: OBJECTIVE: To study the effects of NJY lyophilized powder on physical tolerance and its immune- regulating, antineoplastic effects. METHODS: Loaded-swimming test, hypoxia tolerance test, delayed hypersensitive reaction(DHR) experiment, and macrophage phagocytosis experiments were conducted to investigate the effects of NJY on immune function. By transplanting S180 (1.0×107/ml) sarcoma at the axilla of Kunming mice, the inhibitory effect of NJY on its growth was observed, promotion of the antineoplastic effect of cyclophosphamide, and the possibility of extending mice survival were examined. RESULTS: NJY 16 mg/kg and 64 mg/kg exerted an obvious anti-fatigue effect on mice, with significant difference(P<0.05) from the control group; the survival time of mice with hypoxia in NJY 32 mg/kg group and 64 mg/kg group was significantly increased, with significant difference(P<0.05) from the control group. DHR: swelling extent of mice ear in NJY 32 mg/kg and 64 mg/kg groups was significantly increased compared with the model control group, with significant difference(P<0.05). Macrophage phagocytosis experiment: the phagocytic index of NJY dose groups had no significant difference(P>0.05). The inhibiton rates of NJY dose groups had no significant difference(P>0.05), compared with control group. The life extension rates of NJY dose groups were not prolonged, without significant difference(P>0.05). Combined administration of NJY with cyclophosphamide significantly increased the antineoplastic effect of cyclophosphamide. The inhibition rates were significantly increased compared with the control group after combined administration of cyclophosphamide 60 mg/kg and NJY 16, 32 and 64 mg/kg dose, with significant difference (P<0.05).CONCLUSION:NJY lyophilized powder has immune enhancing effect; it can enhance the anti-fatigue ability of mice and anti-anoxia capacity; markedly enhanced anti-tumor effect of cyclophosphamide.

Key words: NJY lyophilized powder, physical tolerance, immune regulation, antineoplastic effect