反义端粒酶(hTR)基因对人肝癌细胞系生物学行为的影响

doi:10.3969/j.issn.1004-616x.2004.03.005

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2004, Vol. 16 ›› Issue (3): 142-143.doi: 10.3969/j.issn.1004-616x.2004.03.005

Previous Articles Next Articles

Expression of Antisense Human Telomerase RNA Gene in SMMC-7721 Cells

NIE Zhe-qun，LIU Zhong-lin，WANG Wei，et al

Department of General Surgery,Dalian Railway Hospital,Dalian 116001，China

Received:2004-03-07 Revised:2004-04-05 Online:2004-05-30 Published:2004-05-30
Contact: NIE Zhe-qun

Abstract

Abstract: BACKGROOND ＆ AIM:To study the effects of antisense human telomerase RNA (hTR) on SMMC-7721 cells. MATERIAL AND METHODS: The retrovirus vector containing antisense hTR gene was constructed. SMMC-7721 cell line was transfected with antisense hTR expression vector by lipofectamine, and expressions of hTR,telomerase activity in the cells transfected were detected by Southern blot,TRAP－PCR and Flow Cytometry.RESULTS: Clones containing antisense hTR gene were selected by G418.Antisense hTR gene expression was enhanced and the growth of positive clone cells was inhibited after transfection.At last cells containing antisense hTR gene appeared apoptosis. CONCLUSION:Expression of antisense hTR gene significantly inhibited the growth hepatocellular carcinoma cell SMMC-7721,decreased malignant phenotypes and histological atypia.Therefore,hTR gene may be a useful pathway for hepatocellular carcinoma therapy.

Key words: telomerase, antisense, gene therapy, hepatocellular carcinoma

NIE Zhe-qun, LIU Zhong-lin, WANG Wei, et al. Expression of Antisense Human Telomerase RNA Gene in SMMC-7721 Cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2004, 16(3): 142-143.

[1]	ZHU Xinping, JIA Di, LIN Shaohui, LIU Chen, LI Jingyi, ZHAO Weiming. Inhibitory effects of a novel polyoxometalate drug {BiW₈O₃₀}on liver cancer SK-HEP-1 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 47-52,66.
[2]	JIAO Wenjing, ZHANG Jinyan, MA Ming, GUO Xiujuan, FENG Junhua, SHAO Junguo, JIAO Wenpeng. Expression and clinical significance of MTDH and FoxM1 in hepatocellular carcinomas [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 349-353,359.
[3]	LIN Kaihuang, CAI Gaoyang, LIU Xincheng. Effect of mTOR inhibitor AZD2014 on proliferation of hepatoma HCC cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 448-451.
[4]	ZHANG Ju, YE Shulai, ZHANG Changlong, ZHOU Qian. Stimulation of dendritic cell-mediated antitumor tumor effect from HepG2 cell exosomes [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(1): 29-34.
[5]	DING Wenping, LU Yuanyuan, TONG Wenxia, ZHOU Aibin, KONG Xiang, CHEN Yihong. Induction of apoptosis in HepG2 hepatocellular carcinoma cells by chloroquine via the P38MAPK pathway [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(6): 430-434.
[6]	ZHAO Ji, LIU Wenbin, CHEN Hongqiang, LIU Jinyi, YANG Huifang, CAO Jia. Expression of the WFDC1 gene in hepatocellular carcinoma tissues and cell lines and their prognostic values [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(4): 291-296.
[7]	LIU Dan, JIAO Liangbo, ZHANG Wen, WANG Xinyi, CHEN Tao, HU Wei. Effect of Chinese herbal compound QHF on human Huh7 hepatoma cell proliferation and invasion, and expression of surface markers of cancer stem cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(3): 171-175.
[8]	PENG Wei, QIU Fu. Expression and prognostic influence of TM4SF1 in hepatocellular carcinoma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(6): 427-430,436.
[9]	LIU Dezhong, CHANG Bo, LIN Feixiang, LI Xuedong. β-catenin expression in hepatocellular carcinoma and its effect on prognosis [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(4): 300-303.
[10]	HE Jie, WAN Jinghai, LI Xueji, QIAN Haipeng, MENG Xiaoli, HAO Jiajie, XU Xin, WANG Mingrong. TERT promoter mutation is a potential biomarker for predicting poor outcome in gliomas [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(5): 361-365.
[11]	CHEN Jiahong, ZHANG Hang, LAI Yi, JIANG Yan, CHEN Tao. Cell proliferation-related mRNA expression and DNA methylation changes induced by trichloroethylene in the liver and kidney of B6C3F1 male mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(3): 211-215.
[12]	NIU Yong-dong，CHEN Li-ming，WU Man-peng，CHENG He，XU Han，GAO Fen-fei，SHI Gang-gang. Establishment of a HepG2 cell line with stable PXR silencing by lentivirus-mediated RNA interference [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2014, 26(3): 209-212.
[13]	HE Dan-dan，DENG Zhi-hua，YANG Qian. The effect of HBX protein on the proliferation of normal hepatic cells and on telomerase activity [J]. , 2012, 24(3): 183-187.
[14]	MA Dan1, LIU Min2, WANG Ai-ping3, YANG Hua1,*. Effect of COX-2 gene antisense eukaryotic expression vector on SGC-7901 metastasis [J]. , 2011, 23(2): 111-113.
[15]	CHENG Long-qiang, ZHANG Yao, CHEN Chang-jie, YANG Qing-ling, WANG Hui. Expressions of apolipoprotein M, liver receptor homolog-1 and hepatocyte nuclear factor-1α in hepatocellular carcinoma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2010, 22(2): 130-133.

Expression of Antisense Human Telomerase RNA Gene in SMMC-7721 Cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments