Abstract: BACKGROUND ＆ AIM: To investigate the differences of gene expression profile among the pulmonary adenocarcinoma and metastatic lymph node tissues by means of cDNA microarray. MATERIAL AND METHODS: Total RNA was extracted from pulmonary adenocarcinoma and metastatic lymph node tissues in order to get purified mRNA which was retro-transcribed into cDNA. As the probes, the cDNA was labeled with Cy5 and Cy3 fluorescence, then hybridized with the whole human gene chip. The signal image was obtained by Scan Array 4000 fluorescence scanner and analyzed with computer software. RESULTS: 8 genes in 3 subjects with pulmonary adenocarcinoma and metastatic lymph node tissues were identified with common differences in gene expression level, including 5 up-regulated and 3 down-regulated genes. CONCLUSION: The expression levels of 8 genes found both in pulmonary adenocarcinoma and metastatic lymph node tissues were probably related to the development and metastasis of lung cancer.

Key words: pulmonary adenocarcinoma, metastatic lymph nodes, gene expression profile