Carcinogenesis, Teratogenesis & Mutagenesis ›› 2010, Vol. 22 ›› Issue (5): 343-345.doi: 10.3969/j.issn.1004-616x.2010.05.004

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Analysis of differential expression of proteins in portal cholangiocarcinoma cell line FRH 0201 and hepatocellular cell line L02

LIU Bo1, XIAO Xue-yuan2 ,HE Da-cheng2, HUANG Zhi-qiang1   

  1. 1. Department of Hepatobiliary Surgery,General Hospital of People′s Liberation Army, Beijing 100853; 2. College of Life Sciences, Universty Institute for Proteomics, Beijing Normal University, Beijing 100875,China)
  • Received:2010-03-15 Revised:2010-05-30 Online:2010-09-30 Published:2010-09-30
  • Contact: LIU Bo

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Abstract: OBJECTIVE: To analyze the differential expression of proteins in portal cholangiocellular carcinoma cell line FRH 0201 and normal hepatocellular cell line L02, and to screen potential molecular markers for diagnosis of hilar cholangiocarcinoma. METHODS: Surface enhanced laser desorption/ionization (SELDI) mass spectrometry with Proteinchip IMAC3 and WCX2 was performed to compare the expressed proteins in portal cholangiocellular carcinoma cell line FRH 0201 and hepatocellular cell line L02. Protein profiling was examined by PBSII_C Protein Chip Reader and the proteome database was analyzed by Proteinchip Software 3.0.2. RESULTS: 144 protein peaks in FRH 0201 and L02 cell lines were captured by Proteinchip IMAC3 and WCX2，16 differential proteins were found. Four proteins were up_regulated and 12 proteins down_regulated in portal cholangiocellular carcinoma cell line FRH 0201 as compared with L02 cell line. CONCLUSION: The differential proteins could be candidate biomarkers of portal cholangiocarcinoma. The study may contribute to understanding the mechanisms of development of portal cholangiocarcinoma

Key words: portal cholangiocarcinoma, hepatocyte, differential protein