Carcinogenesis, Teratogenesis & Mutagenesis ›› 2004, Vol. 16 ›› Issue (3): 183-184.doi: 10.3969/j.issn.1004-616x.2004.03.018

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A Stable Technique of Maturation in vitro Culture of Human Oocytes

XIE Qing-Dong;ZENG Li-ping;HUNAG Tian-hua   

  1. Research Center for Reproductive Medicine Shantou University Medical College Shantou 515041，China
  • Received:2004-02-27 Revised:2004-04-03 Online:2004-05-30 Published:2004-05-30
  • Contact: HUANG Tian-hua

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Abstract: BACKGROUND & AIM: TO establish a stable in vitro Culture technique of human oocytes． MATERIAL AND METHODS: Immature human oocyte were achieved by puncture follicle and cultured for 24 to 36 hours in HTF medium( containing hMG). RESULTS: Oocytes After Cultured were judged as mature according to the two criteria:the first polar body existed;the cytoplasm was symmetric, light and gramule uniformly． CONCLUSION: The method was stable,this result suggested that the maturation in vitro of immature human oocyte could have great potential for human assisted reproduction．

Key words: oocyte, maturation in vitro culture