hTR基因反义核酸对K562 细胞端粒酶活性和凋亡的影响

›› 2002, Vol. 14 ›› Issue (3): 151-153.doi:

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EFFECT OF ANTISENSE OLIGONUCLEOTIDE AGAINST hTR RNA ON THE TELOMERASE ACTIVITY

HU Jie2ying1, YANG Sheng2li2, JIANG Dong2xia1

1. Henan Tumor Institute, Zhengzhou 450003, China; 2. Henan Medical University, Zhengzhou 450052

Received:2001-10-22 Revised:2002-03-10 Online:2002-07-30 Published:2002-07-30
Contact: HU Jie-ying

Abstract

Abstract: Purpose : To study the inhibitory effect of human telomerase activity and the apoptosis by an antisense oligonucleotide against human telomerase RNA ( hTR) in K562 cells. Methods : Telomerase activity of K562 cells with antisense t reatment was measured using the TRAP assay , and apoptosis was studied by means of TUNEL method. Results : The telomerase activity was effectively inhibited , and the apoptosis was induced by the antisense t reatment . Conclusion : Antisense oligonucleotide against hTR can effectively inhibit the telomerase activity of K562 cells.

Key words: telomerase, K562 cell, antisenset reatment, apoptosis

CLC Number:

HU Jie2ying1, YANG Sheng2li2, JIANG Dong2xia1. EFFECT OF ANTISENSE OLIGONUCLEOTIDE AGAINST hTR RNA ON THE TELOMERASE ACTIVITY[J]. , 2002, 14(3): 151-153.

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EFFECT OF ANTISENSE OLIGONUCLEOTIDE AGAINST hTR RNA ON THE TELOMERASE ACTIVITY

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