Carcinogenesis, Teratogenesis & Mutagenesis ›› 2006, Vol. 18 ›› Issue (5): 404-406.doi: 10.3969/j.issn.1004-616x.2006.05.021

Previous Articles     Next Articles

Optimizing Protocol of Two-Dim-ensional Electrophoresis by Mini Zoom IPG Runner System for the Analysis of Plasma Proteins

ZHOU Hai-Tao1,2, ZHU Hong3, GONG Ying-Hao4, HU Jin-Sen1, GAO Zhou1, JIA Shao-Wei1, LI Ming-Tao2   

  1. 1.Department of Nuclear Medicine，Peking University Shenzhen Hospital, Shenzhen 518036; 2.Proteomics Laboratory, Zhongshan Medical College, Sun Yat-sen University, Guanzhou 510060; 3.Department of Dermatology,No.1 Hospital of China Medical University, Shenyang 110001; 4.Outpatient Treatment Area，Peking University Shenzhen Hospital, Shenzhen 518036,Guangdong,China
  • Received:2006-04-30 Revised:2006-06-12 Online:2006-09-30 Published:2006-09-30
  • Contact: ZHOU Hai-Tao1

PDF (PC)

3272

Abstract: BACKGROUND ＆ AIM: Optimizing the protocol of two-dimensional electrophoresis by Mini Zoom IPG Runner System to analyze plasma proteins. MATERIAL AND MATHODS: Using the immobile IPG strips, ZOOMTM Gels for 2D Electrophoresis and buffer solution system purchased from Invitrogen,two dimensional electrophoresis of plasma proteins was performed.The effect of changing the condition of isoelectric focusing and pretreating the samples,on the two dimensional profiles was analyzed so as to optimize the protocol of the system. RESULTS: Compared with the recommended condition, the profiles were improved by enhancing the voltage of isoelectric focusing, extending the time and desalinizing the samples. CONCLUSION: The protocol of two-dimensional electrophoresis by Mini Zoom IPG Runner System for the analysis of plasma proteins should be optimized by increasing the voltage of isoelectric focusing, extending the time and desalinizing samples.

Key words: plasma proteins, two dimensional electrophoresis, Mini Zoom IPG Runner system