关键词: 全烟气, 气溶胶, Ames试验, 吸烟机

Abstract:

OBJECTIVE: To investigate the in vitro exposure and the genetic toxicity of native whole cigarette smoke，and to provide the basis for mutagenic evaluation of cigarette smoke. METHODS：Using VITROCELL VC10 smoke machine，dilution system and Ames exposure module，whole smoke was examined by Ames assay by adjusting the rate of air and diluted smoke，and the number of cigarette. RESULTS：Under the optimum condition， 5 mL/min diluted smoke and 4 cigarettes，whole smoke showed clear genetic toxicity with 30% S9 mixture and without the overlay agar. CONCLUSION：It was possible to analyse the effects of native whole smoke directly and achieve a dose-dependent induction of revertants. The procedure of exposing bacteria directly to gases and complex mixtures offers new possibilities in the field of inhalation genotoxicology for its evaluation in the Ames assay.

Key words: whole cigarette smoke, aerosol, Ames assay, smoke machine