应用LC/TOF-MS分析食管鳞状细胞癌血浆小分子代谢物的实验研究

doi:10.3969/j.issn.1004-616x.2013.06.002

癌变·畸变·突变

应用LC/TOF-MS分析食管鳞状细胞癌血浆小分子代谢物的实验研究

彭媛,刘冉,李晓波,尹立红*

( 东南大学公共卫生学院环境医学工程教育部重点实验室，江苏南京 210009 )

收稿日期:2013-03-29 修回日期:2013-07-04 出版日期:2013-11-30 发布日期:2013-11-30
通讯作者: 尹立红，E-mail：lhyin@seu.edu.cn
作者简介:彭媛 (1989- )，女，湖南省浏阳市人，硕士研究生，研究方向：环境流行病学。E-mail：pengy0221@sina.com
基金资助:
国家自然科学基金项目(81172747，81072259)；江苏省自然科学基金项目(BK2010407)

A pilot study of plasma metabolic biomarkers for esophageal squamous cell carcinoma by LC/TOF-MS

PENG Yuan，LIU Ran，LI Xiao-bo，YIN Li-hong*

(Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China)

Received:2013-03-29 Revised:2013-07-04 Online:2013-11-30 Published:2013-11-30
Contact: YIN Li-hong，E-mail：lhyin@seu.edu.cn

摘要/Abstract

摘要：

目的： 利用液相色谱-飞行时间质谱 (liquid chromatography-time of flight- mass spectrography，LC/TOF-MS)检测食管鳞状细胞癌 (esophageal squamous cell carcinoma，ESCC)血浆中小分子代谢物质的变化，探讨与ESCC相关的小分子代谢标志物。方法：收集ESCC和健康对照血浆样本各30例，应用LC/TOF-MS方法检测，采用MassHunter软件对数据进行分子特征提取，MPP软件对其进行分析，对可能的代谢物精确质量在METLIN公共数据库中进行搜索查询。结果：与健康对照组相比，ESCC组共找到差异表达的血浆小分子代谢物数目46个，其中13个上调，33个下调。经过METLIN数据库搜索后，ESCC血浆中的磷脂酸、磷脂酰丝氨酸、磷脂酰甘油碎片含量与健康对照组相比显著降低。结论：基于LC/TOF-MS的血浆小分子代谢物分析策略可有效区分食管癌和健康个体，在食管癌的早期诊断和人群筛检方面具有较好的应用前景。

关键词: 代谢组学, 液相色谱-飞行时间质谱, 小分子物质, 食管鳞状细胞癌

Abstract:

OBJECTIVE: The plasma low-molecular weight metabolites of esophageal squamous cell carcinoma were analyzed by liquid chromatography-time of flight- mass spectrography( LC/TOF-MS) to explore potential biomarkers for the early detection of ESCC. METHODS：Plasma samples were obtained from 30 ESCC patients and 30 healthy volunteers，low-molecular weight metabolites were identified by LC/TOF-MS，feature detection was performed by MassHunter software MFE algorithm，statistical analysis was conducted by MPP software，and primary identification was achieved by searching METLIN database. RESULTS：Compared to healthy controls，46 differentially expressed features were detected，13 of which were up-regulated in ESCC group，while the other 33 were down-regulated. After searching with METLIN database，plasma levels of PA，PS，PG were significantly lower in ESCC group. CONCLUSION：ESCC patients are markedly different from healthy people in plasma metabolite composition，and the LC/TOF-MS plasma metabonomics shed light on esophageal cancer early detection and screening.

Key words: metabonomics, liquid chromatography-time of flight-mass spectrography, low-molecular weight metabolites, esophageal squamous cell carcinoma

彭媛,刘冉,李晓波,尹立红. 应用LC/TOF-MS分析食管鳞状细胞癌血浆小分子代谢物的实验研究[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2013.06.002.

PENG Yuan，LIU Ran，LI Xiao-bo，YIN Li-hong. A pilot study of plasma metabolic biomarkers for esophageal squamous cell carcinoma by LC/TOF-MS[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.06.002.

参考文献

[1] Nicholson JK，Lindon JC，Holmes E. “Metabonomics”： understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J]. Xenobiotica，1999， 29 (11)：1181-1189.

[2] Plumb RS，Stumpf CL，Granger JH，et al. Use of liquid chromatography/time-of-flight mass spectrometry and multivariate statistical analysis shows promise for the detection of drug metabolites in biological fluids[J]. Rapid Commun Mass Spectrom， 2003，17 (23)：2632-2638.

[3] Plumb RS，Stumpf CL，Gorenstein MV，et al. Metabonomics： the use of electrospray mass spectrometry coupled to reversed-phase liquid chromatography shows potential for the screening of rat urine in drug development[J]. Rapid Commun Mass Spectrom， 2002，16 (20)：1991-1996.

[4] Denkert C，Budczies J，Kind T， et al. Mass spectrometry-based metabolic profiling reveals different metabolite patterns in invasive ovarian carcinomas and ovarian borderline tumors[J]. Cancer Res，2006，66 (22)：10795-10804.

[5] Ritchie SA，Ahiahonu PWK，Jayasinghe D，et al. Reduced levels of hydroxylated，polyunsaturated ultra long-chain fatty acids in the serum of colorectal cancer patients：implications for early screening and detection[J]. BMC Med，2010，8 (1)：13.

[6] 阿仙姑·哈斯木，巴吐尔·买买提明，伊力亚尔·夏合丁. 不同模式识别方法分析食管癌患者血浆代谢组的磁共振氢谱[J]. 中华肿瘤杂志，2010，32 (9)：681-684.

[7] 杨永霞，梁敏锋，陈阿丽，等. 应用核磁共振代谢组学方法分析食管癌患者血清代谢物[J]. 江苏医药，2010，36 (16)： 1867-1868.

[8] Liscovitch M，Czarny M，Fiucci G，et al. Phospholipase D： molecular and cell biology of a novel gene family[J]. Biochem J，2000，345 (Sup 3)：401-415.

[9] 黄豪博，战榕. 溶血磷脂酸酰基转移酶β与肿瘤关系研究进展[J]. 中国实验血液学杂志，2008，16 (4)：975-978.

[1]	郭秀娟, 杜彦艳, 颜晰, 赵学涛, 韩丽娜, 武一鹏, 王雪晓, 马鸣. CREB在食管鳞癌组织中的表达及其临床意义[J]. 癌变·畸变·突变, 2020, 32(5): 387-390,394.
[2]	赵骅, 陆雪, 蔡恬静, 李爽, 田梅, 刘青杰. 基于代谢组学方法筛选大鼠血浆辐射敏感代谢物[J]. 癌变·畸变·突变, 2020, 32(3): 166-170,176.
[3]	肖瑶, 王美杰, 侯绍英. UPLC/Q-TOF MS/MS技术评价防腐剂硼酸和叠氮化钠对尿液代谢物的影响[J]. 癌变·畸变·突变, 2018, 30(6): 442-446,451.
[4]	陈欣然, 宋永志, 单保恩. 协同刺激分子B7-H4在食管癌前病变组织中的表达及临床意义[J]. 癌变·畸变·突变, 2018, 30(5): 368-373.
[5]	林琳, 范潇予, 李恩灿, 凌亚豪, 臧清策, 林生, 贺玖明, 靳洪涛. 小鼠腘窝淋巴结模型评价3种中药注射液的潜在免疫毒性[J]. 癌变·畸变·突变, 2018, 30(4): 275-278,285.
[6]	陈欣然, 王玲, 王薇, 韩利霞, 刘莉, 单保恩. 4-硝基喹啉-1-氧化物诱导食管鳞状细胞癌发生的研究进展[J]. 癌变·畸变·突变, 2016, 28(2): 151-154.
[7]	刘荟娟，商利，郝佳洁，蒋焱熠，石峰，王春丽，张钰，徐昕，蔡岩，黄大可，贾雪梅，王明荣. AHNAK2蛋白在食管鳞癌中的表达及其临床意义[J]. 癌变·畸变·突变, 2014, 26(1): 36-40.
[8]	张　倩。赵连梅，张建彬，单保恩. 脾酪氨酸激酶与食管鳞状细胞癌的关系[J]. , 2010, 22(6): 423-427.
[9]	董玉然1;王益民2;郭炜1;刘盼1;郭艳丽1;杨植彬1;董稚明1. 包含氧化还原酶的WW结构域基因甲基化与食管鳞状细胞癌的关系[J]. , 2010, 22(2): 91-094.
[10]	杨　霞1/ 杨胜利1/ 吕全军2/ 闫爱华1. 食管鳞状细胞癌中MGMT和p16基因启动子甲基化关系分析[J]. , 2008, 20(4): 254-257.
[11]	雷荣辉，吴纯启，杨保华，马华智，王青秀，廖明阳. 纳米铜经口染毒大鼠血清的代谢组学研究[J]. , 2008, 20(1): 22-027.

应用LC/TOF-MS分析食管鳞状细胞癌血浆小分子代谢物的实验研究

A pilot study of plasma metabolic biomarkers for esophageal squamous cell carcinoma by LC/TOF-MS

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

本文评价