邻苯二甲酸二(2-乙基己)酯对大鼠睾丸和卵巢组织激素相关蛋白表达的影响

doi:10.3969/j.issn.1004-616x.2015.03.014

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (3): 225-229.doi: 10.3969/j.issn.1004-616x.2015.03.014

邻苯二甲酸二(2-乙基己)酯对大鼠睾丸和卵巢组织激素相关蛋白表达的影响

徐新云¹, 秦逍云^1,2, 谭琴^1,2, 柯跃斌¹, 袁建辉¹, 吴德生¹, 刘威¹, 杨晨¹

1. 广东省深圳市疾病预防控制中心, 广东深圳 518055;
2. 深圳大学生命科学学院, 广东深圳 518060

收稿日期:2014-12-30 修回日期:2015-04-01 出版日期:2015-05-30 发布日期:2015-05-30
作者简介:徐新云,E-mail:xyxu2008@163.com
基金资助:
广东省科技计划项目(2013B021800032)

The effects of di-(2-ethylhexyl) phthalate on protein expression of hormonal-related genes in testis and ovary in rats

XU Xinyun¹, QIN Xiaoyun^1,2, TAN Qin^1,2, KE Yuebin¹, YUAN Jianhui¹, WU Desheng¹, LIU Wei¹, YANG Chen¹

1. Shenzhen Center for Disease Control and Prevention, Shenzhen 518055;
2. School of Life Science, Shenzhen University, Shenzhen 518060, Guangdong, China

Received:2014-12-30 Revised:2015-04-01 Online:2015-05-30 Published:2015-05-30

摘要/Abstract

摘要：

目的:探讨邻苯二甲酸二(2-乙基己)酯(DEHP)对大鼠睾丸和卵巢组织激素相关蛋白表达水平的影响。方法:将80只5周龄SD大鼠,雌雄各半,随机分为对照组(玉米油)、DEHP低剂量组(100 mg/kg)、DEHP中剂量组(500 mg/kg)、DEHP高剂量组(1 500 mg/kg),每组雌雄各10只,每周染毒5 d,每天灌胃染毒1次,连续6周。末次染毒24 h后处死动物,提取睾丸或卵巢组织蛋白,应用Western blot分别检测睾丸组织中3β-羟基类固醇脱氢酶(3β-HSD)、促性腺激素释放激素受体(GnRHR)、卵泡刺激素受体(FSHR)及卵巢组织中黄体生成素受体(LHR)和GnRHR的蛋白表达水平。结果:与对照组比较,雄鼠睾丸组织在DEHP 500和1 500 mg/kg剂量时3β-HSD蛋白表达水平均显著升高(P均<0.01);GnRHR蛋白表达水平在1 500 mg/kg剂量组显著下降(P均<0.01);FSHR蛋白表达水平在500和1 500 mg/kg组显著下降(P<0.05或P<0.01)。雌鼠卵巢组织在DEHP 100 mg/kg剂量组时LHR和GnRHR蛋白表达水平与对照组比较差异均无统计学意义(P>0.05),在DEHP 500和1 500 mg/kg剂量组LHR表达水平比对照组下降25%~35%,GnRHR下降60%~80%(P<0.05或P<0.01)。结论:DEHP染毒可引起大鼠睾丸和卵巢组织激素相关蛋白表达水平的改变,干扰大鼠体内性激素的代谢,推测此作用与DEHP的生殖毒性存在密切关系。

关键词: 邻苯二甲酸二(2-乙基己)酯, 3β-羟基类固醇脱氢酶, 促性腺激素释放激素受体, 卵泡刺激素受体, 黄体生成素受体, 生殖毒性

Abstract:

OBJECTIVE: To explore the effects of di-(2-ethylhexyl) phthalate (DEHP) on protein expression of hormonal-related genes including 3β-hydroxysteroid dehydrogenase (3β-HSD), gonadotropin-releasing hormone receptor (GnRHR), follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) in rats. METHODS:A total of 80 5-week-old male and female Sprague-Dawley rats were randomly divided into four groups with 20 rats in each group. The DEHP doses were 0 mg/kg in control group (corn oil), 100 mg/kg in low dose group, 500 mg/kg in medium dose group and 1 500 mg/kg in high dose group. Rats were exposed to DEHP via gavage administration for consecutive six weeks, DEHP administration was once a day and five times a week. After the exposure, rats were anesthetized through ether, then sacrificed for the further studies. The protein expressions of 3β-HSD, GnRHR and FSHR in testis and ovary was analyzed by Western blot. RESULTS:Compared with control group, the expression of 3β-HSD protein was significantly increased in DEHP 500 and 1 500 mg/kg groups ( P<0.01), GnRHR was decreased in DEHP 1 500 mg/kg group (P<0.01), FSHR was decreased in DEHP 500 and 1 500 mg/kg groups (P<0.05 or P<0.01, respectively). The protein expressions of LHR in DEHP 500 and 1 500 mg/kg groups were decreased by 25% to 35% when compared with control, whilst GnRHR protein expressions were decreased by 60% to 80% in DEHP 500 and 1 500 mg/kg groups (P<0.05 or P<0.01). CONCLUSION:DEHP could cause endocrine disorder and interfere with the synthesis of sex hormones in rats, ultimately leading to male and female reproductive dysfunction.

Key words: di-(2-ethylhexyl) phthalate, β-hydroxysteroid dehydrogenase, gonadotropin-releasing hormone receptor, follicle-stimulating hormone receptor, luteinizing hormone receptor, reproductive toxicity

中图分类号:

R994.6

徐新云, 秦逍云, 谭琴, 柯跃斌, 袁建辉, 吴德生, 刘威, 杨晨. 邻苯二甲酸二(2-乙基己)酯对大鼠睾丸和卵巢组织激素相关蛋白表达的影响[J]. 癌变·畸变·突变, 2015, 27(3): 225-229.

XU Xinyun, QIN Xiaoyun, TAN Qin, KE Yuebin, YUAN Jianhui, WU Desheng, LIU Wei, YANG Chen. The effects of di-(2-ethylhexyl) phthalate on protein expression of hormonal-related genes in testis and ovary in rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(3): 225-229.

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邻苯二甲酸二(2-乙基己)酯对大鼠睾丸和卵巢组织激素相关蛋白表达的影响

The effects of di-(2-ethylhexyl) phthalate on protein expression of hormonal-related genes in testis and ovary in rats

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