Notch信号通路相关蛋白在腺性膀胱炎和膀胱癌中的表达与意义

doi:10.3969/j.issn.1004-616x.2018.05.009

癌变·畸变·突变 ›› 2018, Vol. 30 ›› Issue (5): 374-377,383.doi: 10.3969/j.issn.1004-616x.2018.05.009

Notch信号通路相关蛋白在腺性膀胱炎和膀胱癌中的表达与意义

黄壮程, 王宏浩, 沈沛麟, 林明恩, 何学军

汕头大学医学院第一附属医院泌尿外科, 广东汕头 515041

收稿日期:2018-04-23 修回日期:2018-09-03 出版日期:2018-09-30 发布日期:2018-09-30
通讯作者: 何学军,E-mail:Hexjst929@126.com E-mail:Hexjst929@126.com
作者简介:黄壮程,E-mail:huangzc1991@126.com。
基金资助:
广东省医学科研基金（A2018103）；汕头市科技局基金项目（2017026）；广东省自然科学基金（2015A030310078）

Expression and clinical significance of Notch signalingrelated proteins in cystitis glandularis and bladder cancer

HUANG Zhuangcheng, WANG Honghao, SHEN Peilin, LIN Ming'en, HE Xuejun

Department of Urology, The First Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong, China

Received:2018-04-23 Revised:2018-09-03 Online:2018-09-30 Published:2018-09-30

摘要/Abstract

摘要： 目的：探讨Notch信号通路在腺性膀胱炎（CG）及膀胱尿路上皮癌（BUC）发生发展中的作用。方法：选取经病理确诊的CG标本26例，BUC标本50例，其中高级别尿路上皮癌35例，低级别15例；选取正常膀胱组织16例作为对照组。采用免疫组化法分别检测正常膀胱组织、CG组织、BUC组织中Notch1和Hes1蛋白的分布与表达，分析在CG与BUC中Notch1和Hes1表达与临床病理指标的关系。结果：Notch1在CG、BUC组织及正常膀胱组织中均存在表达，阳性表达率分别为65.38%（17/26）、70%（30/50）和25%（4/16）；Hes1在CG、BUC组织及正常膀胱组织中的阳性表达率分别为65.38%（17/26）、76%（38/50）和18.75%（3/13）。CG及BUC组织中Notch1和Hes1蛋白表达均较正常膀胱组织高，差异均有统计学意义（P均 < 0.05），并且Notch1和Hes1的表达强度随BUC病理分级升高而增强（P < 0.05）。结论：Notch1和Hes1与CG的发生及BUC的发展相关，Notch通路蛋白有可能作为判断CG诊治指标之一，并作为BUC侵袭力、复发监测的肿瘤标志物。

关键词: 腺性膀胱炎, 膀胱癌, Notch1, Hes1, 免疫组化

Abstract: OBJECTIVE:To investigate the role of Notch signaling pathway in the development of cystitis glandularis (CG) and bladder urothelial cell carcinoma(BUC). METHODS:Expression of Notch1 and Hes1 protein were assessed by immunohistochemistry in bladder sections of 16 normal bladders and 26 CG samples from 50 patients with BUC. The relationship between CG and BUC,and the pathological grade and clinical stages of BUC were evaluated. RUSULTS:Notch1 was observed in normal bladder,CG samples and BUC samples,and the positive rates were 65.38% (17/26),70% (30/50) and 25% (4/16),respectively. The positive rates of Hes1 in CG,BUC and normal bladder tissues were 65.38% (17/26),76% (38/50) and 18.75% (3/13),respectively. Expressions of Notch1 and Hes1 in BUC and CG samples were significantly higher than that in normal bladder samples (P < 0.05) and increased with the pathological grades (P < 0.05). However,there was no significant difference (P > 0.05) in their expression in BUC and CG samples. CONCLUSION:Our study shows that Notch1 and Hes1 expressions were associated closely with the occurrence and pathological grade of BUC,as well as the occurrence and development of CG. Notch pathway might influence CG for the development of bladder cancer. Therefore,the expression may be used as a biomarker for diagnostic indices and for treatment option of CG.

Key words: cystitis glandularis, bladder cancer, Notch1, Hes1, immunohistochemistry

中图分类号:

R737.14

黄壮程, 王宏浩, 沈沛麟, 林明恩, 何学军. Notch信号通路相关蛋白在腺性膀胱炎和膀胱癌中的表达与意义[J]. 癌变·畸变·突变, 2018, 30(5): 374-377,383.

HUANG Zhuangcheng, WANG Honghao, SHEN Peilin, LIN Ming'en, HE Xuejun. Expression and clinical significance of Notch signalingrelated proteins in cystitis glandularis and bladder cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(5): 374-377,383.

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Notch信号通路相关蛋白在腺性膀胱炎和膀胱癌中的表达与意义

Expression and clinical significance of Notch signalingrelated proteins in cystitis glandularis and bladder cancer

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