Pokemon基因干扰对泌乳素腺瘤细胞的影响及其分子机制

doi:10.3969/j.issn.1004-616x.2016.03.006

癌变·畸变·突变 ›› 2016, Vol. 28 ›› Issue (3): 190-194.doi: 10.3969/j.issn.1004-616x.2016.03.006

Pokemon基因干扰对泌乳素腺瘤细胞的影响及其分子机制

于海洲¹, 唐爱发², 李维平², 刘玉飞², 袁建辉³

1. 深圳市盐田区盐港医院, 广东深圳 518083;
2. 深圳市第二人民医院, 广东深圳 518035;
3. 深圳市疾病预防控制中心, 广东深圳 518055

收稿日期:2015-05-14 修回日期:2015-11-09 出版日期:2016-05-31 发布日期:2016-05-31
通讯作者: 袁建辉,E-mail:jianhui_yuan@126.com E-mail:jianhui_yuan@126.com
作者简介:于海洲,E-mail:296538409@qq.com。
基金资助:
深圳市科技研发项目(JCYJ20130402111321450)

Pokemon gene induced prolactinoma progression and related molecular mechanisms

YU Haizhou¹, TANG Aifa², LI Weiping², LIU Yufei², YUAN Jianhui³

1. Shenzhen Yan'gang Hospital, Yantian District, Shenzhen 518083;
2. No. 2 People's Hospital of Shenzhen, Shenzhen 518035;
3. Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong, China

Received:2015-05-14 Revised:2015-11-09 Online:2016-05-31 Published:2016-05-31

摘要/Abstract

摘要： 目的:探讨原癌基因pokemon干扰对泌乳素腺瘤细胞的影响及其分子机制,为可能的泌乳素腺瘤分子靶向治疗提供依据。方法:从泌乳素腺瘤患者手术切除的肿瘤组织中分离培养原代肿瘤细胞,利用siRNA技术构建pokemon稳定干扰泌乳素腺瘤细胞系(siRNA1、siRNA2和siRNA3),采用荧光定量PCR及Western blot验证pokemon的表达;同时运用CCK8检测pokemon基因干扰对泌乳素腺瘤细胞增殖的影响。采用Western blot检测在泌乳素腺瘤组织及siRNA稳定干扰细胞中pokemon对下游cyclin-A、p14ARF和p21蛋白表达的影响。结果:3株pokemon基因干扰泌乳素腺瘤细胞均构建成功。与空白质粒对照组比较,干扰组的细胞增殖能力均较对照组明显减弱(P均<0.05),且siRNA2干扰效率最高。与正常泌乳素腺组织比较,泌乳素腺瘤组织中pokemon蛋白表达明显增加(P=0.042),而其下游蛋白p14ARF、cyclin-A以及p21表达均显著降低(P均<0.05)。与空白质粒对照组比较,在siRNA2干扰组细胞中p14ARF、cyclin-A和p21蛋白表达水平均明显升高(P均<0.05)。结论:pokemon基因干扰可上调泌乳素腺瘤细胞中肿瘤相关抑制因子的表达,提示pokemon可能作为泌乳素腺瘤治疗的潜在靶位点。

关键词: 泌乳素腺瘤, 原代培养, pokemon, 细胞增殖, cyclin-A, p14ARF, p21

Abstract: OBJECTIVE:Prolactinoma is a common intracranial tumor wihch always leads to serious clinical outcomes. This study aims to confirm the association between the proto-oncogene pokemon and prolactinoma progression, and further explore the molecular mechanisms. METHODS:Tumor tissues were collected from patients with prolactinoma. The primary prolactinoma cells were digested from the tissues and cultured with proper conditions. The expression of pokemon gene was knocked-down by siRNA transfection, and the proliferation of prolactinoma primary cells was analyzed by CCK8 assay. The expression level of pokemon related genes, cyclin-A, p14ARF and p21 in tissues and prolactinoma primary cells were examined by Western blot analysis. RESULTS:Expression of pokemon in prolactinoma primary cell was successfully knocked-down by siRNA transfection. The results showed that siRNA2 showed the highest efficiency of inhibiting the proliferation ability of prolactinoma primary cells. Cyclin-A, p14ARF and p21 were down-regulated by pokemon in both prolactinoma tissues and primary cells. CONCLUSION:Pokemon could improve prolactinoma progression by reducing the expression level of tumor inhibition factors, cyclin-A, p14ARF and p21.

Key words: prolactinoma, primary culture, pokemon, cell progression, cyclin-A, p14ARF, p21

中图分类号:

R736.4

于海洲, 唐爱发, 李维平, 刘玉飞, 袁建辉. Pokemon基因干扰对泌乳素腺瘤细胞的影响及其分子机制[J]. 癌变·畸变·突变, 2016, 28(3): 190-194.

YU Haizhou, TANG Aifa, LI Weiping, LIU Yufei, YUAN Jianhui. Pokemon gene induced prolactinoma progression and related molecular mechanisms[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 190-194.

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Pokemon基因干扰对泌乳素腺瘤细胞的影响及其分子机制

Pokemon gene induced prolactinoma progression and related molecular mechanisms

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