长期暴露的邻苯二甲酸二(2-乙基己基)酯在雌性大鼠体内的代谢及对肝脏的影响

doi:10.3969/j.issn.1004-616x.2017.03.010

癌变·畸变·突变 ›› 2017, Vol. 29 ›› Issue (3): 211-215.doi: 10.3969/j.issn.1004-616x.2017.03.010

长期暴露的邻苯二甲酸二(2-乙基己基)酯在雌性大鼠体内的代谢及对肝脏的影响

董晋, 董新文, 赵跃, 郭纪鹏, 王占举, 那晓琳

哈尔滨医科大学公卫学院环境卫生学教研室, 黑龙江哈尔滨 150081

收稿日期:2016-11-15 修回日期:2017-04-10 出版日期:2017-05-31 发布日期:2017-05-31
通讯作者: 那晓琳,E-mail:naxiaolin1495@sohu.com E-mail:naxiaolin1495@sohu.com
作者简介:董晋,E-mail:1364452419@qq.com。

Prolonged exposure to di(2-ethylhexyl) phthalate on metabolism in female rats

DONG Jin, DONG Xinwen, ZHAO Yue, GUO Jipeng, WANG Zhanju, NA Xiaolin

Department of Environmental Hygiene, School of Public Health, Harbin Medical University, Harbin 150081, Heilongjiang, China

Received:2016-11-15 Revised:2017-04-10 Online:2017-05-31 Published:2017-05-31

摘要/Abstract

摘要： 目的：研究邻苯二甲酸二（2-乙基己基）酯（DEHP）长期暴露后，雌性大鼠体内的代谢情况及对肝脏的影响。方法：将Wistar雌性大鼠64只按体质量随机分为4组，分别为对照（玉米油）组和150、300、600 mg/mL DEHP染毒组，每组16只。采用灌胃方式进行染毒，每天灌胃1次，每次按2.5 μL/g给予，连续灌胃6个月。期间观察大鼠一般生长状况，分别在染毒后3个月和6个月两个时间点采集大鼠尿液。6个月后处死各组大鼠，摘取大鼠的肝、脾和肾称取质量，计算脏体比值；并制备肝组织切片，采用HE染色法观察肝组织形态变化。对采集的2个时间点大鼠尿液，采用高效液相色谱的方法检测尿中DEHP及其代谢物邻苯二甲酸单（乙基己基）酯（MEHP）的含量。结果：与对照组相比，各剂量染毒组大鼠一般生长情况无明显差异，但肝脏的脏体比值增加，HE染色显示300和600 mg/mL DEHP染毒组大鼠肝脏组织轻微病理改变。与对照组相比，3个月时，300和600 mg/mL DEHP组随着染毒剂量的增加，大鼠尿液中DEHP和MEHP含量均明显增加，差异均有统计学意义（P均 < 0.05）。6个月时，600 mg/mL DEHP染毒组大鼠尿液中DEHP和MEHP含量高于其他3组，差异有统计学意义（P < 0.05）。结论：雌性大鼠长期暴露DEHP，体内DEHP和MEHP的残留和蓄积会增加，超过正常的代谢能力，可能会对肝脏造成一定程度的损伤。

关键词: 邻苯二甲酸二(2-乙基己基)酯, 邻苯二甲酸单(乙基己基)酯, 高效液相色谱, 代谢, 肝损伤

Abstract: OBJECTIVE:To study the effect of di(2-ethylhexyl) phthalate (DEHP) on metabolism in livers of female rats. METHODS:64 female Wistar rats were randomly divided into four groups:control (corn oil) and 150,300,600 mg/mL DEHP groups with 16 rats per group. Different doses of DEHP were administered via gavage,daily in volume of 2.5 μL/g,for 6 months. At 3 and 6 months,rat urine samples were collected. Rats were sacrificed at 6 months later. The liver,spleen and kidney were removed,and the ratio of viscera was calculated. Liver tissue sections were prepared and their morphological changes were observed by HE staining. The contents of DEHP and its metabolite,mono-ethylhexyl phthalate (MEHP),in urine were measured by high performance liquid chromatography (HPLC) at 2 time points. The content of MEHP in DEHP and its metabolite,mono-ethylhexyl phthalate,was measured by high performance liquid chromatography (HPLC). RESULTS:Compared with the control group,there was no significant difference in the general growth of the rats in each dose group,but the ratio of the dirty body in the liver increased. HE staining showed slight changes in the liver tissues of the 300 and 600 mg/mL DEHP rats. At 3 months,the levels of DEHP and MEHP in the urine of the 300 and 600 mg/mL DEHP groups were significantly increased with the increase of DEHP dose,the difference was statistically significant (P < 0.05). At 6 months,the levels of DEHP and MEHP in the urine of the 600 mg/mL DEHP group were higher than those of the other three groups (P < 0.05). CONCLUSION:DEHP in DEHP and DEHP and MEHP in the female rats were increased which exceeded the normal metabolic capacity,therefore,the increase might have caused some damage to the liver.

Key words: di(2-ethylhexyl)phthalate, mono-ethylhexyl phthalate, high performance liquid chromatography, metabolism, liver damage

中图分类号:

R944.6

董晋, 董新文, 赵跃, 郭纪鹏, 王占举, 那晓琳. 长期暴露的邻苯二甲酸二(2-乙基己基)酯在雌性大鼠体内的代谢及对肝脏的影响[J]. 癌变·畸变·突变, 2017, 29(3): 211-215.

DONG Jin, DONG Xinwen, ZHAO Yue, GUO Jipeng, WANG Zhanju, NA Xiaolin. Prolonged exposure to di(2-ethylhexyl) phthalate on metabolism in female rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(3): 211-215.

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长期暴露的邻苯二甲酸二(2-乙基己基)酯在雌性大鼠体内的代谢及对肝脏的影响

Prolonged exposure to di(2-ethylhexyl) phthalate on metabolism in female rats

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