维生素E琥珀酸酯处理人胃癌细胞过程中自噬与内质网应激的交互作用

doi:10.3969/j.issn.1004-616x.2015.03.001

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (3): 161-167.doi: 10.3969/j.issn.1004-616x.2015.03.001

• 论著 • 下一篇

维生素E琥珀酸酯处理人胃癌细胞过程中自噬与内质网应激的交互作用

宋华翠¹, 侯丽颖¹, 綦峥^2,3, 吴坤¹

1. 哈尔滨医科大学公共卫生学院营养与食品卫生学教研室, 黑龙江哈尔滨 150081;
2. 哈尔滨商业大学生命科学与环境科学研究中心, 黑龙江哈尔滨 150076;
3. 国家教育部抗肿瘤天然药物工程研究中心, 黑龙江哈尔滨 150076

收稿日期:2015-01-08 修回日期:2015-03-05 出版日期:2015-05-30 发布日期:2015-05-30
通讯作者: 吴坤,E-mail:wukun_15000@126.com E-mail:wukun_15000@126.com
作者简介:宋华翠,E-mail:songhuacui0615@163.com
基金资助:
国家自然科学基金资助项目(81172651)

Interaction between endoplasmic reticulum stress and autophagy induced by vitamin E succinate in human gastric carcinoma cells

SONG Huacui¹, HOU Liying¹, QI Zheng^2,3, WU Kun¹

1. Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin 150081;
2. Materia Medica Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076;
3. Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin 150076, Heilongjiang, China

Received:2015-01-08 Revised:2015-03-05 Online:2015-05-30 Published:2015-05-30

摘要/Abstract

摘要：

目的:探讨维生素E琥珀酸酯(VES)处理人胃癌SGC-7901细胞过程中自噬与内质网应激的交互作用。方法:用不同剂量(0、5、10、15、20 μg/mL)VES处理人胃癌SGC-7901细胞24 h后,激光共聚焦显微镜观察细胞内自噬标志蛋白微管相关蛋白l轻链3(LC3)荧光强度和分布情况,Western blot分别检测内质网应激标志物葡萄糖调节蛋白78(GRP78)、葡萄糖调节蛋白94(GRP94)和LC3、Beclin-1的表达情况;在分别加入自噬抑制剂3-甲基腺嘌呤(3-MA)和氯喹(CQ)以及内质网应激抑制剂4-苯基丁酸(4-PBA)预处理2 h后,以20 μg/mL VES处理人胃癌SGC-7901细胞24 h,Western blot分别检测内质网应激标志物GRP78、GRP94及自噬标志蛋白LC3、Beclin-1的变化。结果:与阴性对照组比较,随着VES剂量的增加,激光共聚焦显微镜观察到LC3在细胞内点状分布逐渐聚集和增强,GRP78和GRP94表达先降低后升高(P均<0.05),LC3和Beclin-1表达逐渐升高(P均<0.05);VES+3-MA组及VES+CQ组的GRP78和GRP94蛋白表达均高于单独VES组(P均<0.05);VES+4-PBA组的LC3和Beclin-1蛋白表达均低于单独VES组(P均<0.05)。结论:VES处理人胃癌细胞过程中自噬与内质网应激可以相互调节,具有交互作用。

关键词: 维生素E琥珀酸酯, 内质网应激, 自噬, 胃癌细胞

Abstract:

OBJECTIVE: To discuss the interaction between autophagy and endoplasmic reticulum stress in human gastric cancer SGC-7901 cells treated with vitamin E succinate (VES). METHODS:Human gastric cancer SGC-7901 cells were treated with VES of different doses (0, 5, 10, 15, 20 μg/mL), then after 24 h laser confocal microscope was used to study fluorescence intensity and distribution of the autophagy marker protein microtubule-associated protein 1 light chain 3 (LC3). Western blot was used to evaluate the level of endoplasmic reticulum stress markers glucose regulative protein 78(GRP78), glucose regulative protein 94(GRP94) and LC3, Beclin-1. SGC-7901 cells were pre-treated with autophagy inhibitors, 3-methyladenine(3-MA) and chloroquine (CQ), inhibitor of endoplasmic reticulum stress, 4-phenylbutyric acid(4-PBA), then human gastric cancer SGC-7901 cells were treated with 20 μg/mL VES for 24 h. Western blot evaluated the level of GRP78, GRP94, LC3 and Beclin-1. RESULTS:With increasing doses of VES, the LC3 in cells began to gather and intensity, GRP78 and GRP94 were decreased, then increased again and peaked at 24 h. The levels of GRP78 and GRP94 in VES+3-MA group and VES+CQ group were significately higher compared with VES alone (all P<0.05). The levels of LC3 and Beclin-1 in VES+4-PBA group were higher compared with VES alone(both of P<0.05). CONCLUSION:In VES-treated human gastric cancer cells, the autophagy and endoplasmic reticulum stress could interact and regulate each other.

Key words: vitamin E succinate, endoplasmic reticulum stress, autophagy, gastric cancer cells

中图分类号:

R730.2

宋华翠, 侯丽颖, 綦峥, 吴坤. 维生素E琥珀酸酯处理人胃癌细胞过程中自噬与内质网应激的交互作用[J]. 癌变·畸变·突变, 2015, 27(3): 161-167.

SONG Huacui, HOU Liying, QI Zheng, WU Kun. Interaction between endoplasmic reticulum stress and autophagy induced by vitamin E succinate in human gastric carcinoma cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(3): 161-167.

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维生素E琥珀酸酯处理人胃癌细胞过程中自噬与内质网应激的交互作用

Interaction between endoplasmic reticulum stress and autophagy induced by vitamin E succinate in human gastric carcinoma cells

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