TGF-β1对UVB辐射诱导人角质形成细胞miRNA和环氧合酶-2表达的影响及机制

doi:10.3969/j.issn.1004-616x.2019.05.001

癌变·畸变·突变 ›› 2019, Vol. 31 ›› Issue (5): 341-346.doi: 10.3969/j.issn.1004-616x.2019.05.001

• 论著 • 下一篇

TGF-β1对UVB辐射诱导人角质形成细胞miRNA和环氧合酶-2表达的影响及机制

李玉文, 李辰, 封江彬, 范莉, 刘建香, 刘青杰, 田梅

中国疾病预防控制中心辐射防护与核安全医学所, 辐射防护与核应急中国疾病预防控制中心重点实验室, 北京 100088

收稿日期:2019-04-04 修回日期:2019-09-20 出版日期:2019-09-30 发布日期:2019-10-09
通讯作者: 田梅,E-mail:tianmei@nirp.chinacdc.cn E-mail:tianmei@nirp.chinacdc.cn
作者简介:李玉文,E-mail:liyuwen@nirp.chinacdc.cn。

Effect of UVB irradiation on TGF-β1,and expression of miRNA and cyclooxygenase-2 in human keratinocytes

LI Yuwen, LI Chen, FENG Jiangbin, FAN Li, LIU Jianxiang, LIU Qingjie, TIAN Mei

Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China

Received:2019-04-04 Revised:2019-09-20 Online:2019-09-30 Published:2019-10-09

摘要/Abstract

摘要： 目的：研究TGF-β1对UVB诱导的人角质形成细胞（HaCaT）中miRNA及炎症反应蛋白环氧合酶-2（COX-2）表达的影响，探讨TGF-β1与紫外辐射诱导的细胞损伤、炎症反应、肿瘤发生等的关系。方法：将10 ng/μL的TGF-β1加入HaCaT细胞培养基中，作用48 h后，利用实时荧光定量PCR检测0、10、20、30 mJ/cm₂ UVB照射后6 h，以及30 mJ/cm₂ UVB照射不同时间（4、12和24 h）后HaCaT细胞内miRNA-23a和let-7c的表达水平；采用Western blot方法检测30 mJ/cm₂ UVB照射不同时间（0、2、4、8、12、24和48 h）后HaCaT细胞中COX-2和caspase-3蛋白表达水平的变化。结果：TGF-β1预先处理可诱导HaCaT细胞在UVB照射后，细胞中miR-23a的表达增加，let-7c的表达受到抑制；TGF-β1预先作用细胞后，COX-2的表达水平明显增加（P < 0.05），活化的caspase-3亚基自照射后12 h开始随时间延长而减少。结论：TGF-β可以调节紫外线照射后HaCaT细胞miRNAs的表达水平，诱导COX-2的表达增加，在细胞增殖和浸润、炎症反应及细胞抗凋亡中发挥作用。

关键词: 转化生长因子-β, UVB, 微小RNA, 环氧合酶-2, 炎症

Abstract: OBJECTIVE:To study the effect of TGF-β1 on expression of miRNA and the inflammatory protein,cyclooxygenase-2 (COX-2),in human keratinocytes (HaCaT) after UVB irradiation. METHODS:10 ng/μL of TGF-β1 was added to cultured HaCaT cells for 48 h and then irradiated with UVB:0,10,20,30 mJ/cm₂ UVB and different time (4,12 and 24 h) after 30 mJ/cm₂ UVB irradiation. Real-time fluorescent quantitative PCR was used to detected expression levels of miRNA-23a and let-7c 6 h after irradiation. Expression levels of COX-2 and caspase-3 were detected by Western blot at different time (0,2,4,8,12,24 and 48 h) after 30 mJ/cm₂ UVB irradiation. RESULTS:Expression of miR-23a was increased and let-7c was generally down-regulated. Expression of COX-2 was significantly increased (P < 0.05),and cleaved-caspase3 decreased gradually at 12-48 h after irradiation. CONCLUSION:The data indicate that TGF-β could regulate the expression levels of miRNA and COX-2 which have been shown to play a role in cell proliferation,infiltration,inflammation and anti-apoptosis.

Key words: TGF-β, UVB, miRNA, COX-2, inflammation

中图分类号:

R811.5

李玉文, 李辰, 封江彬, 范莉, 刘建香, 刘青杰, 田梅. TGF-β1对UVB辐射诱导人角质形成细胞miRNA和环氧合酶-2表达的影响及机制[J]. 癌变·畸变·突变, 2019, 31(5): 341-346.

LI Yuwen, LI Chen, FENG Jiangbin, FAN Li, LIU Jianxiang, LIU Qingjie, TIAN Mei. Effect of UVB irradiation on TGF-β1,and expression of miRNA and cyclooxygenase-2 in human keratinocytes[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 341-346.

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TGF-β1对UVB辐射诱导人角质形成细胞miRNA和环氧合酶-2表达的影响及机制

Effect of UVB irradiation on TGF-β1,and expression of miRNA and cyclooxygenase-2 in human keratinocytes

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