关键词: 染发类化妆品, 染发剂, 细菌回复突变试验, 致突变性

Abstract: OBJECTIVE: To investigate mutagenic activities of 178 hair dyeing cosmetics using the bacterial reverse mutation test.METHODS: Mutagenicity of 178 hair dyeing cosmetics was detected by referring to the bacterial reverse mutation assay method in "Safety and Technical Standards for Cosmetics" (2015 edition).RESULTS: Among the 178 hair dyeing cosmetics,the positive rate of bacterial reverse mutation assay was 19.67%(35/178) with S₉ added;the positive rate was 3.93%(7/178) without S₉.Among the 35 mutagenic hair dye,35 were positive for TA98,and 34 and 31 were positive for 5 and 10 mg/dish with S₉ added.Without S₉,the number of positive tests at 2.5 and 5 mg/dish was 7 and 5,respectively.The number of positive detections of TA97a was 15.Under the condition of adding S₉,the number of positive detections at 5,10,and 20 mg/dish were 7,14,and 8,respectively.CONCLUSION: Among the 178 hair dyeing cosmetics,the numbers of positive detections of TA98 and TA97a were relatively large,and the positive doses mostly appeared in the 10 mg/dish,5 mg/dish (+S₉),and 2.5 mg/dish (-S₉).

Key words: hair dyeing cosmetics, hair dyes, bacterial reverse mutation assay, mutagenicity