离子液体1-庚基-3-甲基咪唑氯盐对小鼠的亚急性毒性

doi:10.3969/j.issn.1004-616x.2023.06.006

癌变·畸变·突变 ›› 2023, Vol. 35 ›› Issue (6): 439-444,451.doi: 10.3969/j.issn.1004-616x.2023.06.006

离子液体1-庚基-3-甲基咪唑氯盐对小鼠的亚急性毒性

陈世怿¹, 夏柱南¹, 柳鑫^1,2, 杨庆¹

1. 武汉轻工大学食品科学与工程学院, 湖北武汉 430023;
2. 湖北省疾病预防控制中心应用毒理湖北省重点实验室, 湖北武汉 430079

收稿日期:2023-07-04 修回日期:2023-10-12 出版日期:2023-11-30 发布日期:2023-12-09
通讯作者: 杨庆
作者简介:陈世怿，E-mail：chenshiyi423@126.com。
基金资助:
国家自然科学基金(22006118)；应用毒理湖北省重点实验室开放课题项目(AT-2021-03)

Sub-acute toxicity of ionic liquid 1-heptyl-3-methylimidazolium chloride in mice

CHEN Shiyi¹, XIA Zhunan¹, LIU Xin^1,2, YANG Qing¹

1. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023;
2. Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Center for Disease Control and Prevention, Wuhan 430079, Hubei, China

Received:2023-07-04 Revised:2023-10-12 Online:2023-11-30 Published:2023-12-09

摘要/Abstract

摘要： 目的：探究1-庚基-3-甲基咪唑氯盐(C₇[MIM]Cl)对小鼠的亚急性毒性。方法：根据C₇[MIM]Cl对小鼠的半数致死剂量(LD₅₀)119.00 mg/kg，将24只SPF级昆明小鼠随机分为对照组，低(1/50 LD₅₀，2.38 mg/kg)、中(1/20 LD₅₀，5.95 mg/kg)和高(1/10 LD₅₀，11.9 mg/kg)剂量C₇[MIM]Cl染毒组，每组6只，每天灌胃1次，连续灌胃28 d，对照组灌胃给予等体积的生理盐水。记录小鼠摄食量和体质量变化情况，实验结束取小鼠心、肝、脾、肺、肾和卵巢，称取脏器质量并计算脏器系数，检测血清生化指标，并采用HE染色观察小鼠各脏器的病理变化。结果：与对照组比较，5.95 mg/kg C₇[MIM]Cl染毒组小鼠饲料消耗量降低(P=0.05)；血清生化指标表明2.38 mg/kg C₇[MIM]Cl染毒组小鼠血清中谷丙转氨酶活性显著上升(P=0.049)，而5.95 mg/kg组小鼠血清总胆红素、血尿素、尿酸和总胆固醇以及11.9 mg/kg组小鼠血糖水平显著下降(P<0.05)；组织切片观察结果显示不同剂量C₇[MIM]Cl亚急性染毒对小鼠的肝、脾、肺、肾以及卵巢均有一定程度的损伤，其中肝脏和肾脏损伤更为明显。结论：C₇[MIM]Cl亚急性染毒可引起小鼠肝脏和肾脏的功能性损伤。

关键词: 1-庚基-3-甲基咪唑氯盐, 小鼠, 亚急性毒性, 肝脏, 肾脏

Abstract: OBJECTIVE：To investigate sub-acute toxicity of the ionic liquid，1-heptyl-3-methy- limidazolium chloride，(C₇[MIM]Cl) in mice. METHODS：According to the median lethal dose (LD₅₀) of C₇[MIM]Cl to mice as 119.00 mg/kg， Kunming mice (n=24) were randomly divided into blank control group and low，middle and high dose (1/50 LD₅₀，1/20 LD₅₀ and 1/10 LD₅₀) groups (n=6 per group). Mice were treated with C₇[MIM]Cl once a day by gavage for 28 days. The control group was given an equal volume of normal saline by gavage. The changes of food intake and body mass of mice were recorded；organ coefficients were calculated；serum biochemical indexes were detected；and the pathological changes of each organ were observed by H&E staining. RESULTS：The food intake in middle exposure group was lower than the control group (P=0.05). Serum biochemical indices showed that the activities of alanine aminotransferase (ALT) in the liver of the low exposure group was significantly increased (P=0.049). The total bilirubin (TBIL)，UREA，uric acid (UA) and cholesterol (CHO) in the serum of the middle exposure group was significantly lower than the control (P<0.05). The glucose (GLU) level in the high dose group was significantly decreased (P<0.05). The observation of tissue section showed that different exposure doses induced a certain degree of damage to liver，spleen，lung，kidney and ovary. Especially，damages to the liver and kidney were more obvious. CONCLUSION：With sub-acute exposure of C₇[MIM]Cl，liver and kidney were important target organs for damage from C7[MIM]Cl.

Key words: C₇[MIM]Cl, mice, sub-acute toxicity, liver, kidney

中图分类号:

R114

陈世怿, 夏柱南, 柳鑫, 杨庆. 离子液体1-庚基-3-甲基咪唑氯盐对小鼠的亚急性毒性[J]. 癌变·畸变·突变, 2023, 35(6): 439-444,451.

CHEN Shiyi, XIA Zhunan, LIU Xin, YANG Qing. Sub-acute toxicity of ionic liquid 1-heptyl-3-methylimidazolium chloride in mice[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2023, 35(6): 439-444,451.

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离子液体1-庚基-3-甲基咪唑氯盐对小鼠的亚急性毒性

Sub-acute toxicity of ionic liquid 1-heptyl-3-methylimidazolium chloride in mice

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