黄曲霉毒素检测方法的研究进展

doi:10.3969/j.issn.1004-616x.2024.05.014

摘要/Abstract

摘要： 黄曲霉毒素(AFT)是来源于黄曲霉和寄生曲霉等真菌的次级代谢产物。AFT污染会直接或间接影响人和动物的生命健康，导致严重的经济损失。通常需要检测AFT的样本包括粮食作物、食品以及生物样本等，其中生物样本包括细胞、肝脏、尿液等。本文就黄曲霉毒素的检测方法进行综述，并分析各方法检测样本种类的异同及其优劣。黄曲霉毒素有较多种类，目前受到关注的主要是黄曲霉毒素B1(AFB1)和黄曲霉毒素M1(AFM1)。在食品领域AFB1的检测方法开发较多；在乳制品中AFM1的检测方法也较多；但生物样本中的AFB1检测方法较少，已开发的方法主要为高效液相色谱法。生物样本的前处理方式复杂是限制黄曲霉毒素检测方法开发的一个重要原因，本综述可为进一步开发和优化针对不同样本尤其是生物样本中的AFB1检测方法提供参考。

关键词: 黄曲霉毒素, 检测方法, 黄曲霉毒素B1, 黄曲霉毒素M1

中图分类号:

R991

罗绣, 孙震晓. 黄曲霉毒素检测方法的研究进展[J]. 癌变·畸变·突变, 2024, 36(5): 417-420.

参考文献

[1] NAZHAND A, DURAZZO A, LUCARINI M, et al. Characteristics, occurrence, detection and detoxification of aflatoxins in foods and feeds[J]. Foods, 2020, 9(5): 644.
[2] 吴坤坦, 齐德生. 黄曲霉毒素B1在乳畜中的转化[J]. 饲料工业, 2018, 39(20): 59-64.
[3] KORTEI N K, ANNAN T, KYEI-BAFFOUR V, et al. Exposure assessment and cancer risk characterization of aflatoxin M₁(AFM₁) through ingestion of raw cow milk in southern Ghana[J]. Toxicol Rep, 2022, 9: 1189-1197.
[4] GRAMANTIERI L, GNUDI F, VASURI F, et al. Aflatoxin B1 DNA-adducts in hepatocellular carcinoma from a low exposure area[J]. Nutrients, 2022, 14(8): 1652.
[5] MIN L, FINK-GREMMELS J, LI D G, et al. An overview of aflatoxin B1 biotransformation and aflatoxin M1 secretion in lactating dairy cows[J]. Anim Nutr, 2021, 7(1): 42-48.
[6] ALAMERI M M, KONG A S, ALJAAFARI M N, et al. Aflatoxin contamination: an overview on health issues, detection and management strategies[J]. Toxins, 2023, 15(4): 246.
[7] 邵坤. 基于适配体纳米磁珠的黄曲霉毒素B1萃取及检测方法研究[D]. 成都: 西华大学, 2019.
[8] KUSSAK A, ANDERSSON B, ANDERSSON K. Immunoaffinity column clean-up for the high-performance liquid chromatographic determination of aflatoxins B1, B2, G1, G2, M1 and Q1 in urine[J]. J Chromatogr B Biomed Appl, 1995, 672(2): 253-259.
[9] 樊文研, 宋嵩文, 杨焕威. 柱后衍生高效液相色谱法测定食用植物油中的黄曲霉素B1的含量[J]. 中国药师, 2017, 20(3): 591-593.
[10] MANEEBOON T, CHUAYSRINULE C, MAHAKARNCHANAKUL W. Optimization and validation of dispersive liquid-liquid microextraction for simultaneous determination of aflatoxins B1, B2, G1, and G2 in Senna leaves and pods using HPLC-FLD with pre-column derivatization[J]. Toxins, 2023, 15(4): 277.
[11] 刘恒, 王安虎, 刘晓燕. 苦荞籽中黄曲霉毒素B1质量分数检测方法的研究[J]. 西昌学院学报: 自然科学版, 2021, 35(4): 6-9.
[12] PANDEY A K, SHAKYA S, PATYAL A, et al. Detection of aflatoxin M1 in bovine milk from different agro-climatic zones of Chhattisgarh, India, using HPLC-FLD and assessment of human health risks[J]. Mycotoxin Res, 2021, 37(3): 265-273.
[13] LIU R M, DING Y X, LI W N, et al. Protective role of curcumin on broiler liver by modulating aflatoxin B1-induced DNA methylation and CYPs expression[J]. Ecotoxicol Environ Saf, 2023, 260: 115086.
[14] WEI L L, CHEN Y N, SHAO D L, et al. Determination of aflatoxins in milk by PS-MWCNT/OH composite nanofibers solid-phase extraction coupled with HPLC-FLD[J]. Molecules, 2023, 28(16): 6103.
[15] 马海艳. 食品药品中痕量真菌毒素检测方法的研究与应用[D]. 石家庄: 河北医科大学, 2018.
[16] DEMIRHAN B E, DEMIRHAN B. Investigation of twelve significant mycotoxin contamination in nut-based products by the LC-MS/MS method[J]. Metabolites, 2022, 12(2): 120.
[17] VICTOR JEYARAJ S V, LOY M J, GOH K W, et al. Aflatoxin tests in herbal products and its quantification: latest updates[J]. Front Nutr, 2022, 9: 956077.
[18] MEHTA R, SHETTY S A, YOUNG M F, et al. Quantification of aflatoxin and ochratoxin contamination in animal milk using UHPLC-MS/SRM method: a small-scale study[J]. J Food Sci Technol, 2021, 58(9): 3453-3464.
[19] ZHOU Y F, XIONG S C, ZHANG K K, et al. Quantum bead-based fluorescence-linked immunosorbent assay for ultrasensitive detection of aflatoxin M₁ in pasteurized milk, yogurt, and milk powder[J]. J Dairy Sci, 2019, 102(5): 3985-3993.
[20] SUMON A H, ISLAM F, MOHANTO N C, et al. The presence of aflatoxin M₁ in milk and milk products in Bangladesh[J]. Toxins, 2021, 13(7): 440.
[21] JAYARATNE W M S C, ABEYRATNE A H M A K, DE ZOYSA H K S, et al. Detection and quantification of Aflatoxin B1 in corn and corn-grown soils in the district of Anuradhapura, Sri Lanka[J]. Heliyon, 2020, 6(10): e05319.
[22] NARDO F D, CAVALERA S, BAGGIANI C, et al. Enzyme immunoassay for measuring aflatoxin B1 in legal Cannabis[J]. Toxins, 2020, 12(4): 265.
[23] PECORELLI I, GUARDUCCI N, VON HOLST C, et al. Critical comparison of analytical performances of two immunoassay methods for rapid detection of aflatoxin M1 in milk[J]. Toxins, 2020, 12(4): 270.
[24] 李细芬, 毛雯雯, 张雅琴, 等. 6种中药材黄曲霉毒素B1快速检测方法研究[J]. 中国现代中药, 2018, 20(8): 968-974.
[25] 蔡芬. 改进型免疫侧向层析黄曲霉毒素B1检测方法研究[D], 合肥: 合肥工业大学, 2020.
[26] WANG H Q, LIU M J, ZHANG Y M, et al. Rapid detection of Aspergillus flavus and quantitative determination of aflatoxin B1 in grain crops using a portable Raman spectrometer combined with colloidal Au nanoparticles[J]. Molecules, 2022, 27(16): 5280.
[27] FENG Y T, HE L, WANG L, et al. Detection of aflatoxin B₁ based on a porous anodized aluminum membrane combined with surface-enhanced Raman scattering spectroscopy[J]. Nanomaterials, 2020, 10(5): 1000.
[28] CHEN B H, INBARAJ B S. Recent trends in analysis of mycotoxins in food using carbon-based nanomaterials[J]. J Food Drug Anal, 2022, 30(4): 562-589.
[29] GABBITAS A, AHLBORN G, ALLEN K, et al. Advancing mycotoxin detection: multivariate rapid analysis on corn using surface enhanced Raman spectroscopy (SERS)[J]. Toxins, 2023, 15(10): 610.
[30] 章琼. 计算机辅助黄曲霉素B1分子印迹SERS传感器的制备与应用研究[D]. 上海: 上海师范大学, 2021.
[31] 林佳. 浅谈生物传感器法检测婴幼儿米粉中黄曲霉毒素B1含量[J]. 食品安全导刊, 2019(18): 98-99.