LncRNA PCA3在甲基丙烯酸环氧丙酯诱导16HBE恶性转化细胞中的表达及意义

doi:10.3969/j.issn.1004-616x.2016.03.005

Abstract

Abstract: OBJECTIVE:To investigate the induction of expression of long non-coding RNA PCA3(LncRNA PCA3) by glycidyl methacrylate (GMA) in malignantly transformed 16HBE cells. METHODS:Malignantly trans-formed 16HBE cells (treated with 8 μg/mL GMA) and solvent control cells (DMSO) of the 30^th generations were harvested. High throughput LncRNA microarray was used to detect differences in expression profile of LncRNAs among the two groups of cells. Changes in LncRNAs were screened through strategies such as fold change and neighboring genes analysis. Real-time fluorescence quantitative PCR (qPCR) and gene chip were applied to measure the expression levels of LncRNA PCA3 and PRUNE2, respectively. RESULTS:Based on the result of LncRNA microarrays, LncRNA PCA3 in GMA-treated cells was up-regulated by 7.17 folds while PRUNE2 was down-regulated by 2.54 folds. QPCR showed that the expression of LncRNA PCA3[(2.67±0.63)×10^-5] in GMA-treated cells was significantly higher than that in the solvent control cells[(1.36±0.44)×10^-5] (P<0.05). On the other hand, the gene chip analyses showed that expression of PRUNE2(10.95) was reduced compared to the solvent control cells(19.46). CONCLUSION:LncRNA PCA3 expression can be considered as one of the stable and specific biomarkers involved in GMA- malignant transformation of 16HBE cells.

Key words: glycidyl methacrylate, human bronchial epithelial cells, LncRNA PCA3, PRUNE2

CLC Number:

R994.6

LIU Hongmei, WANG Quankai, XIE Guangyun, WEN Yanan, XU Jianning. Induction of LncRNA PCA3 by glycidyl methacrylate in malignant transformed 16HBE cells and its significance[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 185-189.

References

[1] Bianco T,Chenevix-Trench G,Walsh DC,et al. Tumor specific distribution of BRCA1 promoter region methylation supports a pathogenetic role in breast and ovarian cancer[J]. Carcinogenesis,2000,21(2):147-151.
[2] Takamochi K,Oh S,Matsuoka J,et al. Clonality status of multifocal lung adenocarcinomas based on the mutation patterns of EGFR and K-ras[J]. Lung Cancer,2012,75(3):313-320.
[3] Caley DP,Pink RC,Trujillano D,et al. Long non-coding RNAs,chromatin,and development[J]. Sci World J,2010,8(10):90-102.
[4] Bussemakers MJ,van Bokhoven A,Verhaegh GW,et al. DD3:a new prostate-specific gene,highly overexpressed in prostate cancer[J]. Cancer Res,1999,59(23):5975-5979.
[5] 杨敏,许建宁,王全凯,等. 甲基丙烯酸环氧丙酯致人支气管上皮细胞恶性转化研究[J]. 中华预防医学杂志,2009,43(3):187-192.
[6] 董琳,胡洁,王全凯,等. 甲基丙烯酸环氧丙酯致人支气管上皮细胞恶性转化过程中相关基因表达变化的研究[J]. 癌变·畸变·突变,2010,22(4):249-254.
[7] Chris PP,Peter LO,Wolf R. Evolution and functions of long noncoding RNAs[J]. Cell,2009,136(4):629-641.
[8] Lipovich L,Johnson R,Lin CY. MacroRNA underdogs in a microRNA world:Evolutionary,regulatory,and biomedical significance of mammalian long non-protein-coding RNA[J]. Biochimica et Biophysica Acta,2010,1799(9):597-615.
[9] Prasanth KV,Spector DL. Eukaryotic regulatory RNAs:an answer to the ‘genome complexity’ conundrum[J]. Genes Dev,2007,21(1):11-42.
[10] Hessels D,Klein GJ,van Oort I,et al. DD3PCA3-based molecular urine analysis for the diagnosis of prostate cancer[J]. Eur Urol,2003,44(1):8-16.
[11] Xu C,Yang M,Tian J,et al. Malat-1:a long non-coding RNA and its important 3' end functional motif in colorectal cancer metastasis[J]. Int J Oncol,2011,39:169-175.
[12] Knudson AG Jr. Mutation and cancer:Statistical study of retinoblastoma[J]. Proc Natl Acad Sci USA,1971,68(4):820-823.
[13] Cavenee WK, Dryja TP,Phillips RA,et al. Expression of recessive alleles by chromosomal mechanisms in retinoblastoma[J]. Nature,1983,305(5937):779-784.
[14] Salameh A,Lee AK,Cardó-Vila M,et al. PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3[J]. Proc Natl Acad Sci USA,2015,112(27):8403-8408.
[15] Soh UJ,Low BC. BNIP2 extra long inhibits RhoA and cellular transformation by Lbc RhoGEF via its BCH domain[J]. J Cell Sci,2008,121(10):1739-1749.
[16] Machida T,Fujita T,Ooo ML,et al. Increased expression of proapoptotic BMCC1,a novel gene with the BNIP2 and Cdc42GAP homology (BCH) domain,is associated with favorable prognosis in human neuroblastomas[J]. Oncogene,2006,25(13):1931-1942.
[17] LR Zhao,W Tian,GW Wang,et al. The prognostic role of PRUNE2 in leiomyosarcoma[J]. Chin J Cancer,2013,32(12):648-652.

Induction of LncRNA PCA3 by glycidyl methacrylate in malignant transformed 16HBE cells and its significance

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Xinwei, WANG Quankai, MA Shunpeng, WANG Miao, WUHAN Baolier, GU Yiting, KANG Tongying, XU Jianning. Long non-coding RNA expression analysis and ceRNA regulatory network in glycidyl methacrylate-induced malignant transformation of 16HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 1-6.
[2]	WANG Miao, WANG Quankai, LI Xinwei, MA Shunpeng, WUHAN Baolier, XU Jianning. m⁶A methylation of mRNAs in glycidyl methacrylate-induced malignant transformation of 16HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(6): 405-409.
[3]	MA Shunpeng, WANG Quankai, WANG Miao, SONG Jiayang, WUHAN Baolier, XIE Guangyun, XU Jianning. Long non-coding RNA regulates apoptosis during glycidyl methacrylate-induced malignant transformation of 16HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 1-5,31.
[4]	CAI Ying, LI Runbing, ZHENG Kai, QIN Shuangjian, LI Boru, ZHANG Zhaohui, XU Xinyun. Differential microRNAs expression and bioinformatics analyses in HBE cells exposed to PM_2.5 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(5): 355-362.
[5]	CAI Ying, ZHENG Kai, QIN Shuangjian, LI Boru, YU Shuyuan, LIU Ning, JI Jiajia, ZHANG Zhaohui, XU Xinyun. Silencing of c-fos gene on expression of oncogenes and apoptosis genes in HBE cells exposed to PM_2.5 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(4): 298-303,308.
[6]	WANG Bingyu, CAI Ying, ZHENG Kai, XIE Hongwei, XU Xinyun. Effect of PM_2.5 on expression of genes related to carcinogenesis and mutagenesis in HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(1): 33-38,42.
[7]	XIE Guangyun, GUO Haoran, WANG Quankai, MA Shunpeng, SONG Jiayang, WUHAN Baolier, XU Jianning. Differential expressions of LINC00472 during glycidyl methacrylate-induced malignant transformation of 16HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 449-453.
[8]	WANG Bingyu, ZHENG Kai, XU Xinyun, XIE Hongwei, YU Junhui, LONG Dingxin. PM_2.5 on DNA damage in human bronchial epithelial cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 469-473,497.
[9]	WANG Quankai, XIE Guangyun, MA Shunpeng, GUO Haoran, WUHAN Baolier, SONG Jiayang, XU Jianning. Genotoxicity evaluation of glycidyl methacrylate [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 479-482.
[10]	WANG Quankai, WANG Boshen, XIE Guangyun, KANG Tongying, ZHU Baoli, XU Jianning. Expression of LncRNA EMX2OS during glycidyl methacrylate-induced malignant transformation of 16HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(6): 422-426.
[11]	WANG Quankai, XIE Guangyun, LIU Hongmei, XU Jianning. METTL9 methylation status in malignant transformation of 16HBE cells induced by glycidyl methacrylate [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 432-436.
[12]	CHEN Wen-tao，FAN Yan-feng，ZHANG Hui-tao，YANG Jin. Change of cell cycle in human bronchial epithelial cells and epithelioid lung fibroblasts exposed to benzo (a) pyrene [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(6): 454-456.
[13]	LI Huan-huan1，WANG An-na1，WANG Quan-kai，TAN Feng，XU Jian-ning. Study on the anti-oncogene of P15 methylation status in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(1): 18-21.
[14]	WANG An-na，DONG Lin，WANG Quan-kai，HU Jie，XU Jian-ning. Changes of TGF-β signal pathway in the process of 16HBE malignant transformation induced by glycidyl methacrylate [J]. , 2012, 24(2): 91-95.
[15]	LI Ying1，WANG Quan-kai1，2，WANG An-na1，2，HU Jie1，XU Jian-ning1，2，*. Expressing the alteration of DNA repair genes in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate [J]. , 2011, 23(3): 161-165.