甲基丙烯酸环氧丙酯诱导16HBE细胞恶性转化过程中LncRNA表达特征分析及ceRNA调控网络预测

doi:10.3969/j.issn.1004-616x.2022.01.001

Abstract

Abstract: OBJECTIVE: To investigate differential expression of long non-coding RNA (LncRNA) during glycidyl methacrylate (GMS)-induced malignant transformation of human bronchial epithelial 16HBE cells,and to explore the regulatory effect of LncRNA on cell function and related signaling pathways at different stages of malignant transformation. METHODS: 16HBE cells were divided into GMA treatment group and DMSO control group,and the cells of the two groups were collected respectively. LncRNA microarray was used to detect and analyze differences of LncRNA expression in the malignantly transformed 16HBE cells. The key differentially expressed LncRNAs were screened out using TargetScan and MiRanda databases. Cytoscape was used to construct a ceRNA regulatory network with differentially expressed LncRNAs as the core. After that,the target genes and pathways of differentially expressed LncRNA were analyzed and predicted,and the relative expression levels of the above LncRNA were detected and verified by real-time fluorescence quantitative PCR. RESULTS: Microarray screening results showed that,compared with DMSO group,16 LncRNAs in GMA group were up-regulated in the process of the malignant transformation. On this basis,a LncRNA-related ceRNA regulatory network consisting of 3 LncRNAs,32 mRNAs and 204 miRNAs was constructed. Three regulatory axes in the network were significantly correlated with GMA-induced malignant transformation of 16HBE cells (P<0.05),and this showed that LncRNA G013234-hsa-miR-378b-TMEM129,LncRNA CTA-384D8.35-hsa-miR-486-3p-LYPD,LncRNA G087116-miR-29b-3p-NAV1 were closely associated with the malignant transformation process ceRNA triples. Results of qPCR showed that relative expression levels of LncRNA G013234,CTA-384D8.35 and G087116 in different cell stages were consistent with results of LncRNA microarray. CONCLUSION: During GMA-induced malignant transformation of 16HBE cells,LncRNA G013234,CTA-384D8.35 and G087116 were up-regulated at different stages of transformation. Construction of the ceRNA regulatory network and key mRNAs related to cell malignant transformation formed the theoretical basis for the process of GMA-induced malignant transformation of 16HBE cells.

Key words: glycidyl methacrylate, malignant transformation, LncRNA, ceRNA network

CLC Number:

R994.6

LI Xinwei, WANG Quankai, MA Shunpeng, WANG Miao, WUHAN Baolier, GU Yiting, KANG Tongying, XU Jianning. Long non-coding RNA expression analysis and ceRNA regulatory network in glycidyl methacrylate-induced malignant transformation of 16HBE cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 1-6.

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Long non-coding RNA expression analysis and ceRNA regulatory network in glycidyl methacrylate-induced malignant transformation of 16HBE cells

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