三氯生的体外遗传毒性评价

doi:10.3969/j.issn.1004-616x.2018.01.014

Abstract

Abstract: OBJECTIVE: Genotoxicity of triclosan was evaluated in TK6 and bacterial cells by alkaline comet assay,cytokinesis-block micronucleus (CBMN) test and bacterial reverse mutation test (Ames test). METHODS: Comet assay and CBMN test were performed by TK6 cell,and 5 doses of TCS were set as 3.5,8.8,17.5,26.3 and 35 μmol/L. Three Salmonella typhimurium tester strains TA98,TA100 and YG7108 (DNA repair deficient strain,Ogt^-/Ada^-) were employed to perform Ames test,and 8 doses of TCS were set at 0.000 5,0.001 67,0.005,0.016 7,0.05,0.167,0.5 and 1.67 μg/plate. RESULTS: The results of comet assay showed that TCS caused DNA damage with a significant increase in comets tail length,tail DNA intensity and tail moment (P < 0.01),and have a statistical correlation with tail DNA intensity (r=0.943,P=0.017). The CBMN test indicated that TCS did not induce increased frequency of micronucleus (P > 0.05) and no consistent increase in numbers of revertant colonies of three strains was seen in Ames test (P > 0.05). CONCLUSION: TCS mainly induced severe DNA injury to TK6 cell in a dose-dependent manner. TCS did not cause chromosome damage and also was not mutagenic to three Salmonella typhimurium strains.

Key words: triclosan, comet assay, micronucleus test, Ames test, genotoxicity

CLC Number:

R994.6

CAO Yiyi, XI Jing, TANG Weifeng, YOU Xinyue, LIU Weiying, LUAN Yang. Genotoxicity evaluation of triclosan in vitro[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 71-75.

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Genotoxicity evaluation of triclosan in vitro

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