›› 2011, Vol. 23 ›› Issue (4): 263-268.doi: 10.3969/j.issn.1004-616x.2011.04.005

• 论著 • 上一篇    下一篇

子宫内膜癌耐药细胞B-MD-C1(ADR+/+) 与非耐药细胞B-MD-C1 的蛋白质差异研究

张 超, 武立华, 艾 军, 单保恩*   

  1. 河北医科大学第四医院科研中心 ,河北石家庄 050011
  • 收稿日期:2011-02-22 修回日期:2011-05-09 出版日期:2011-07-30 发布日期:2011-07-30
  • 通讯作者: 单保恩

Identification of differentially expressed proteins in B-MD-C1 (ADR+/+) and B-MD-C1 by proteomics

ZHANG Chao,WU Li-hua,AI jun,SHAN Bao-en*   

  1. Research Center, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, Hebei, China
  • Received:2011-02-22 Revised:2011-05-09 Online:2011-07-30 Published:2011-07-30
  • Contact: SHAN Bao-en

摘要: 目的: 比较子宫内膜癌耐药细胞B-MD-C1 (ADR+/+)与非耐药细胞B-MD-C1的蛋白质表达谱,识别和鉴定差异表达蛋白,分析差异蛋白与其耐药的关系。 方法:采用双向凝胶电泳及基质辅助激光解吸电离-飞行时间质谱法对B-MD-C1 (ADR+/+)与B-MD-C1细胞进行蛋白质分离和表达谱研究,利用生物信息学方法鉴定差异蛋白质。 结果:B-MD-C1细胞与B-MD-C1 (ADR+/+)相比,蛋白质表达差异>2倍的蛋白质点有35个,其中18个蛋白点表达上调,17个蛋白点表达下调。应用Mascot软件查询Swiss-prot数据库初步鉴定差异表达的蛋白质点24个,这些蛋白质可能是B-MD-C1细胞产生耐药的相关蛋白,包括细胞骨架蛋白、代谢酶类、细胞周期相关蛋白、信号转导和凋亡调控相关蛋白、蛋白合成和参与糖酵解的蛋白等。 结论:热休克蛋白、β-微管蛋白、丙酮酸激酶同工酶、核糖体蛋白等是B-MD-C1(ADR+/+)与B-MD-C1细胞间的差异蛋白,这些蛋白多与B-MD-C1(ADR+/+)细胞耐药密切相关。

关键词: 子宫内膜癌, 蛋白质组学, 多药耐药性, 质谱分析

Abstract: OBJECTIVE: Multidrug resistance (MDR) is a major obstacle for effective treatment of tumors. Previous studies of MDR tended to concentrate in a single or a few related genes and their protein products. We focused on the total protein with the method of proteomics. The aim of this study was to investigate differently expressed protein in B-MD-C1 and B-MD-C1 (ADR+/+) cells by proteomic analysis. METHODS:The holoproteins of human carcinoma of endometrium cell lines B-MD-C1 and B-MD-C1 (ADR+/+) were measured by two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). RESULTS:Thirty-five differential proteins were analyzed by peptide mass fingerprinting. The differentially expressed proteins could be divided into six groups based on their functions:molecular chaperones,cystoskeleton proteins(actin-related protein 3,lamin-B1 and tubulin beta chain),metabolic enzymes (78 kDa glucose-regulated protein,retinal dehydrogenase),proteins associated with cell cycle (eukaryotic initiation factor 4A-III),proteins associated with cell proliferation,differentiation and apoptosis (endoplasmic reticulum protein,endoplasmic reticulum protein),and proteins associated with carbohydrate metabolism (60S acidic ribosomal protein,alpha-enolase). CONCLUSION:These differentially expressed proteins provided some clues to the mechanism of tumor cell resistant to cisplatin,providing the basis of searching for potential target of carcinoma.

Key words: carcinoma of endometrium, comparative proteomics, multidrugs resistance, mass spectrometry analysis