ERCC2/XPD基因缺失对苯并[a]芘所致DNA损伤修复的影响

doi:10.3969/j.issn.1004-616x.2012.06.001

癌变·畸变·突变

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ERCC2/XPD基因缺失对苯并[a]芘所致DNA损伤修复的影响

肖莎/刘秋芳/徐韬钧/关阳阳/靳翠红/李丹丹/逯晓波*

中国医科大学公共卫生学院卫生毒理教研室，辽宁沈阳 110001

收稿日期:2012-07-16 修回日期:2012-09-25 出版日期:2011-11-30 发布日期:2011-11-30
通讯作者: LU Xiao-bo，E-mail：xblu@mail.cmu.edu.cn
作者简介:肖莎 (1986- )，女，湖北人，硕士研究生，研究方向：遗传毒理学。
基金资助:
国家自然科学基金 (30972506)

Effect of deficient ERCC2/XPD gene on the repair of DNA damage induced by benzo[a]pyrene

XIAO Sha，LIU Qiu-fang，XU Tao-jun，GUAN Yang-yang，JIN Cui-hong，LI Dan-dan，LU Xiao-bo*

Hygiene Toxicology Department, Public Health School, China Medical University, Shenyang 110001, China

Received:2012-07-16 Revised:2012-09-25 Online:2011-11-30 Published:2011-11-30
Contact: LU Xiao-bo，E-mail：xblu@mail.cmu.edu.cn
About author:肖莎 (1986- )，女，湖北人，硕士研究生，研究方向：遗传毒理学。
Supported by:
国家自然科学基金 (30972506)

摘要/Abstract

摘要：

目的：探讨核苷酸切除修复交叉互补基因2 (excision repair cross complementation group 2/Xeroderma pigmentosum D，ERCC2/XPD)在苯并[a]芘所诱导的细胞DNA损伤与修复过程中的作用。方法：应用中国仓鼠卵巢细胞系CHO野生型AA8和ERCC2表达缺失型UV5作为细胞对照模型，MTT法比较两种细胞经苯并[a]芘处理后细胞抑制率的差别；彗星试验和Rad51免疫荧光试验检测不同浓度苯并[a]芘处理及修复24 h后细胞DNA损伤修复的情况。结果：与野生型AA8细胞相比，UV5细胞对苯并[a]芘所致损伤更加敏感，细胞存活率降低 (P<0.05)。彗星试验和Rad51免疫荧光试验结果显示，UV5细胞由于缺失ERCC2/XPD基因，修复苯并[a]芘所致DNA损伤能力降低 (P<0.05)。结论：ERCC2/XPD蛋白在核苷酸切除修复中发挥解旋作用，对苯并[a]芘所致DNA损伤修复至关重要。

关键词: 核苷酸切除修复交叉互补基因2, 苯并[a]芘, 核苷酸切除修复, AA8, UV5

Abstract:

OBJECTIVE: To investigate ERCC2/XPD (excision repair cross complementation group 2/Xeroderma pigmentosum D) function in the repair of DNA damage induced by benzo[a]pyrene. METHODS：China hamster ovary (CHO) cell line including wild type AA8 and ERCC2/XPD defective mutant UV5，was set up as a cell contrast model. DNA damage levels at different time points after benzo[a]pyrene treatment were evaluated by MTT cell inhibition assay，modified comet assay and Rad51 immunofluorescence test. RESULTS：UV5 was more sensitive to benzo[a]pyrene when compared with AA8. The DNA damage caused by benzo[a]pyrene was repaired by AA8 but not by UV5. CONCLUSION：ERCC2/XPD，as an important helicase in nucleotide excision repair，could play a critical role in repairing DNA damage induced by benzo[a]pyrene.

Key words: ERCC2/XPD, benzo[a]pyrene, nucleotide excision repair, AA8, UV5

肖莎/刘秋芳/徐韬钧/关阳阳/靳翠红/李丹丹/逯晓波*. ERCC2/XPD基因缺失对苯并[a]芘所致DNA损伤修复的影响[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2012.06.001.

XIAO Sha，LIU Qiu-fang，XU Tao-jun，GUAN Yang-yang，JIN Cui-hong，LI Dan-dan，LU Xiao-bo*. Effect of deficient ERCC2/XPD gene on the repair of DNA damage induced by benzo[a]pyrene[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2012.06.001.

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ERCC2/XPD基因缺失对苯并[a]芘所致DNA损伤修复的影响

Effect of deficient ERCC2/XPD gene on the repair of DNA damage induced by benzo[a]pyrene

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