毒死蜱和乐果联合染毒对大鼠胆碱酯酶活性的影响

doi:10.3969/j.issn.1004-616x.2013.01.007

癌变·畸变·突变

毒死蜱和乐果联合染毒对大鼠胆碱酯酶活性的影响

吴惠,乙楠楠,李亭亭,何贤松,赵敏娴,姚欣雅,王灿楠*

( 东南大学公共卫生学院环境医学工程教育部重点实验室，东南大学公共卫生学院营养与食品卫生系，江苏南京 210009 )

收稿日期:2012-09-19 修回日期:2012-11-28 出版日期:2013-01-30 发布日期:2013-01-30
通讯作者: 王灿楠
作者简介:吴惠(1987- )，女，江苏宝应人，硕士研究生，研究方向：食品安全风险评估。
基金资助:
国家自然科学基金资助项目(81072304)

Combined toxic effect of chorpyriphos and dimethoate on cholinesterase in rats

WU Hui，YI Nan-nan，LI Ting-ting，HE Xian-song，ZHAO Min-xian，YAO Xin-ya，WANG Can-nan*

(Key Laboratory of Medical Engineering Environment, Nutrition and Food Hygiene Department, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China)

Received:2012-09-19 Revised:2012-11-28 Online:2013-01-30 Published:2013-01-30
Contact: WANG Can-nan，E-mail：wcnseu@126.com

摘要/Abstract

摘要：

目的：研究毒死蜱和乐果联合染毒对大鼠血清和脑组织乙酰胆碱酯酶 (acetylcholinesterase，AChE)和丁酰胆碱酯酶 (butyrylcholinesterase，BuChE)活性的影响。方法：采用3×3析因设计，雌性SD大鼠按体质量随机分为9个剂量组和1个阴性对照组，每组10只。选用毒死蜱和乐果的无可见有害作用水平 (no observed adverse effect level，NOAEL)、1/100半数致死剂量 (median lethal dose，LD50)和1/50 LD50作为低、中、高3个剂量水平，分别为毒死蜱0.03、1.35、2.70 mg/ (kg·d)，乐果1.20、3.25、6.50 mg/ (kg·d)。灌胃染毒30 d，染毒结束后，经乙醚麻醉，股动脉取血，脱臼处死，剥取大脑皮质和海马，使用胆碱酯酶试剂盒测定胆碱酯酶活性。结果：染毒30 d后，高剂量组大鼠胆碱酯酶活性低于对照组 (P＜0.05)；析因设计方差分析显示，毒死蜱和乐果联合染毒，对大鼠血清和皮质AChE活性无交互影响；对海马AChE和血清、脑组织BuChE活性存在交互作用 (P＜0.01)。结论：经口染毒高剂量有机磷农药可显著抑制血清和脑组织的胆碱酯酶活性，两种有机磷农药混配后可对胆碱酯酶活性产生交互作用。

关键词: 毒死蜱, 乐果, 乙酰胆碱酯酶, 丁酰胆碱酯酶, 联合作用, 析因分析

Abstract:

OBJECTIVE: To study the combined toxic effect of chorpyriphos and dimethoate on plasma and brain cholinesterase activities in female rats. METHODS：3×3 factorial analysis was used in this experiment. Adult female SD rats were randomly divided into 10 groups of ten rats (including a control group and nine treated groups) according to their weight. The NOAEL (no observed adverse effect level)，1/100 LD50 (median lethal dose)and 1/50 LD50 of chlorpyrifos and dimethoate were selected as the low，medium，and high dose level，respectively. The dose of chlorpyrifos were 0.03，1.35，2.70 mg/ (kg·d) respectively，and those of dimethoate were 1.20，3.25，6.50 mg/ (kg·d) respectively. Blood was collected through arteria cruralis after 30 days of treatment via gastric gavage，then rats were killed and tissues including cortex and hippocampus were collected. Cholinesterase activity was estimated by the Pureauto CHE. RESULTS：The difference of ChE activity between high dose group and control group was significant. No interaction was noted between chorpyriphos and dimethoate on plasma and cortex AChE. The combination of chorpyriphos and dimethoate caused an interactive effect on plasma and brain BuChE inhibition and hippocampus AChE inhibition (P＜0.01). CONCLUSION：High dose combination of organophosphorus pesticides could significantly inhibit the activity of ChE in blood and brain，and also lead to an interactive effect on plasma and brain ChE inhibition.

Key words: chorpyriphos, dimethoate, acetylcholinesterase, butyrylcholinesterase, combined effect, factorial analysis

吴惠,乙楠楠,李亭亭,何贤松,赵敏娴,姚欣雅,王灿楠. 毒死蜱和乐果联合染毒对大鼠胆碱酯酶活性的影响[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2013.01.007.

WU Hui，YI Nan-nan，LI Ting-ting，HE Xian-song，ZHAO Min-xian，YAO Xin-ya，WANG Can-nan. Combined toxic effect of chorpyriphos and dimethoate on cholinesterase in rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.01.007.

参考文献

[1] 李钦云，赵铃铃. 有机磷农药对食品的污染防治[J]. 工业卫生与职业病，2005，31 (4)：260- 263.

[2] Abdollahi M，Mostafalou S，Pournourmohammadi S，et al. Oxidative stress and cholinesterase inhibition in saliva and plasma of rats following subchronic exposure to malathion[J]. Comp Biochem Phys C，2004，137 (1)：29-34.

[3] 何凤生，陈署旸. 混配农药中毒的防治研究[J].中华医学信息导报，2002 ，(11)：16- 17.

[4] Amitai G，Moorad D，Adani R. Inhibition of acetylcholinesterase and butyrylcholinesterase by chlopyrifosoxon[J]. Biochem Parmacol，1998， 56：293-299.

[5] Wang QD，Chen HQ，Li FZ. Distribution of the scavenger esterases[J]. Chin J Parmacol Toicol，1998，12 (2)：91-93.

[6] 杨玉林，芮振荣，王宏，等. 气相色谱法分析尿样中六种有机磷农药代谢产物[J]. 中国卫生检验杂志，2003，13 (1)：24-26.

[7] 孙运光，周志俊，顾祖维. 有机磷农药生物标志物的研究进展[J]. 劳动医学，2000，17 (1)：58-60.

[8] 郑捷，周志俊，张雪梅，等. 慢性有机磷农药接触羧基脂酶活力的影响[J]. 工业卫生与职业病，2005，31 (2)：68-71.

[9] 周志俊. 基础毒理学[M]. 上海：复旦大学出版社，2008：6.

[10] Luis CC，Gisela K，NoemíR，et al. Binary mixtures of azinphos-methyl oxon and chlorpyrifos oxon produce in vitro synergistic cholinesterase inhibition in planorbarius corneus[J]. Chemosphere， 2012，88：450-458.

[11] Timchalk C，Poet TS，Hinman MN，et al. Pharmacokinetic and pharmacodynamic interaction for a binary mixture of chlorpyrifos and diazinon in the rat[J]. Toxicol Appl Pharmacol，2005，205 (1)：31-42.

[12] 文一. 有机磷农药的联合毒性及其毒理学机理研究[D]. 北京：中国农业科学院，2008：27-28.

毒死蜱和乐果联合染毒对大鼠胆碱酯酶活性的影响

Combined toxic effect of chorpyriphos and dimethoate on cholinesterase in rats

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