精子相关抗原9在膀胱移行细胞癌中的表达及临床意义

doi:10.3969/j.issn.1004-616x.2013.01.008

癌变·畸变·突变

精子相关抗原9在膀胱移行细胞癌中的表达及临床意义

冯元元,何学军*,杨峰涛,蔡聪,佘绍逸

( 汕头大学医学院第一附属医院，广东汕头 515041 )

收稿日期:2012-10-05 修回日期:2012-11-16 出版日期:2013-01-30 发布日期:2013-01-30
通讯作者: 何学军
作者简介:冯元元 (1985- )，女，河南商丘市人，硕士研究生，研究方向：膀胱癌发病机制。

Expression and clinical significance of sperm-associated antigen 9 in transitional cell carcinoma of the bladder

FENG Yuan-yuan，HE Xue-jun*，YANG Feng-tao，CAI Cong，SHE Shao-yi

(Department of Urology, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong, China)

Received:2012-10-05 Revised:2012-11-16 Online:2013-01-30 Published:2013-01-30
Contact: HE Xue-jun，E-mail：hexjst929@126.com

摘要/Abstract

摘要：

目的：探讨精子相关抗原9 (sperm-associated antigen 9，SPAG9)在膀胱移行细胞癌 (bladder transitional cell carcinoma， BTCC)中的表达并分析其临床意义。方法：选取经临床病理诊断的BTCC标本98例，正常膀胱组织30例，应用免疫组化染色方法检测SPAG9在BTCC中的表达和分布情况，分析其与肿瘤的临床分期、病理分级及2年复发率的关系。结果：SPAG9在正常膀胱组织中为阴性表达，而在BTCC组中的阳性表达率为75.5% (74/98)，并且随着肿瘤分期和分级的升高SPAG9蛋白的表达增加，SPAG9阳性表达率在病理分级G1、G2与G3级之间的差异有统计学意义 (P<0.05)；在临床分期Ta与T1组间的差异有统计学意义 (P<0.05)； SPAG9蛋白表达阳性的非肌层浸润性膀胱癌患者，其2年复发率显著高于阴性表达者 (P<0.05)。结论：SPAG9蛋白在BTCC中呈高表达状态，可成为BTCC有意义的肿瘤标志物，并有可能作为判断 BTCC侵袭力、监测复发的重要指标。阻断SPAG9表达有望成为膀胱癌靶向治疗的新靶点。

关键词: 膀胱移行细胞癌, SPAG9, 免疫组化

Abstract:

OBJECTIVE: To investigate the expression of sperm-associated antigen 9 (SPAG9) in bladder transitional cell carcinoma (BTCC)，and analyze its clinical significance. METHODS：The expression of SPAG9 protein was assessed by immunohistochemistry in bladder sections of 98 patients with BTCC and 30 normal bladders. The relationship between SPAG9 and grade of pathology，clinical state and the 2-year recurrence rate were analyzed. RESULTS：No obvious expression of SPAG9 was observed in normal bladder samples. However BTCC samples showed high expression ratio of SPAG9，reaching 75.5% (74/98). Furthermore，the expression level of SPAG9 increased with tumor stage and grade. SPAG9 positive expression rates showed significant difference between histological grades G1，G2 and G3 (P<0.05)；and clinical stages Ta and T1 group (P<0.05). Besides，non-muscle-invasive bladder cancer patients with positive SPAG9 expression showed high incidence of 2-year recurrence. CONCLUSION：SPAG9 is high expressed in BTCC，making it a useful tumor marker and a possible indicator for estimating the invasiveness and recurrence of BTCC. Blocking SPAG9 expression and its functions may become targeted therapy of bladder cancer treatment.

Key words: bladder transitional cell carcinoma, sperm-associated antigen 9, immunohistochemistry

冯元元,何学军,杨峰涛,蔡聪,佘绍逸. 精子相关抗原9在膀胱移行细胞癌中的表达及临床意义[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2013.01.008.

FENG Yuan-yuan，HE Xue-jun，YANG Feng-tao，CAI Cong，SHE Shao-yi. Expression and clinical significance of sperm-associated antigen 9 in transitional cell carcinoma of the bladder[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.01.008.

参考文献

[1] Ghoneim MA，Abdel-Latif M，Mekresh M，et al. Radical cystectomy for carcinoma of the bladder：2，720 consecutive cases 5 years later[J]. J Urol ，2008，180 (1)：121-127.

[2] Shariat SF，Karakiewicz PI，Palapattu GS，et al. Outcomes of radical cystectomy for transitional cell carcinoma of the bladder：A contemporary series from the bladder cancer research consortium[J]. J Urol，2006，176 (6 Sup 1)：2414-2422.

[3] Garg M，Kanojia D，Khosla A，et al. Sperm-associated antigen 9 is associated with tumor growth，migration，and invasion in renal cell carcinoma[J]. Cancer Res，2008，68 (20)：8240-8248.

[4] Garg M，Kanojia D，Salhan S，et al. Sperm-associated antigen 9 is a biomarker for early cervical carcinoma[J]. Cancer， 2009， 115 (12)：2671-2683.

[5] Peng Yu，Lei Yan，Hui Zhang，et al. Expression and clinical significance of sperm-associated antigen 9 in patients with endometrial carcinoma[J]. Internat J Gynecol Cancer，2012，22 (1)：87-93.

[6] Jagadish N，Rana R，Selvi R，et al. Characterization of a novel human sperm-associated antigen 9 (SPAG9) having structural homology with c-Jun N-terminal kinase-interacting protein[J]. Biochem J，2005，389 (Sup 1)：73–82.

[7] Shankar S，Mohapatra B，Suri A. Cloning of a novel human testis mRNA specifically expressed in testicular haploid germ cells，having unique palindromic sequences and encoding a leucine zipper dimerization motif[J]. Biochem Biophys Res Commun，1998，243 (2)：561–565.

[8] Qi M，Elion EA. MAP kinase pathways[J]. J Cell Sci，2005， 118 (16)：3569–3572.

[9] Kim M，Murakami A，Kawabata K，et al. Epigallocatechin-3-gallate promotes pro-matrix metalloproteinase-7 production via activation of the JNK1/2 pathway in HT-29 human colorectal cancer cells[J]. Carcinogenesis，2005，26 (9)：1553–1562.

[10] Collins CS，Hong J，Sapinoso L，et al. A small interfering RNA screen for modulators of tumor cell motility identifiesMAP4K4 as a pro-migratory kinase[J]. Proc Natl Acad Sci USA， 2006， 103 (10)：3775–3780.

[11] Hernandez S，Lopez-Knowles E，Lioreta J，et al. Prospective study of FGFR3 mutations as a prognostic factor in non-muscle-invasive urothelial bladder carcinomas[J]. Eur Urol ，2008，54 (4)：835–843.

[12] Zendman AJ，Ruiter DJ，Van-Muijen GN. Cancer/testis-associated genes：identification，expression profile，and putative function[J]. J Cell Physiol，2003，194 (3)：272-288.

[13] Garg M，Chaurasiya D，Rana R，et al. Sperm-associated antigen 9，a novel cancer testis antigen，is a potential target for immunotherapy in epithelial ovarian cancer[J]. Clin Cancer Res，2007，13 (5)：1421-1428.

[14] Wei CL，Wu Q，Vega SV，et al. A global map of p53 transcription-factor binding sites in the human genome[J]. Cell， 2006，124 (1)：207-219.

[15] Bernardini S，Billerey C，Martin M，et a1. The predictive value of muscularis mucosae invasion and p53 over expression on progression of stage T1 bladder carcinoma[J]．J Urol， 2001，165 (1)：42-46，526-528．

[16] Kanojia D，Garg M，Gupta S，et a1. Sperm associated antigen 9，a novel biomarker for early detection of breast cancer[J]. Cancer Epidemiol Biomarkers Prev，2009，18 (2)：630-639.

[17] Scudellari M. A ballsy search for cancer targets[J]. Nat Med， 2011，17 (8)：916-918.

精子相关抗原9在膀胱移行细胞癌中的表达及临床意义

Expression and clinical significance of sperm-associated antigen 9 in transitional cell carcinoma of the bladder

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价

[1]	朱　斌/ 杨罗艳/ 赵晓昆. 膀胱癌多药耐药基因MDR_1与金属硫蛋白_3表达的关系[J]. , 2007, 19(5): 412-415.
[2]	熊术道李景荣温博贵李冠武李　璟. 人膀胱移行细胞癌EGFR基因片段与核基质蛋白的相关性[J]. , 2007, 19(2): 106-109.