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Survivin、NF-кB和STAT3 mRNA在宫颈癌中的表达及其临床意义

常玉珍1,李秀梅2,封  敏3,高  崎1,*   

  1. 新疆维吾尔自治区中医医院妇科,新疆  乌鲁木齐  830099
  • 收稿日期:2013-06-05 修回日期:2014-03-06 出版日期:2014-05-30 发布日期:2014-05-30
  • 通讯作者: 高 崎,E-mail:1009808852@qq.com;Tel:0991-5812557
  • 作者简介:常玉珍(1962- ),女,汉族,新疆人,副主任医师,研究方向:妇科肿瘤的发病机制。

The expression and significance of Survivin,NF-κB and STAT3 mRNA in cervical cancer

CHANG Yu-zhen1,LI Xiu-mei2,FENG Min3,GAO Qi1,*   

  1. Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830099
  • Received:2013-06-05 Revised:2014-03-06 Online:2014-05-30 Published:2014-05-30

摘要:

目的: 检测Survivin、NF-κB、STAT3 mRNA在40例子宫颈癌组织及30例正常子宫颈组织中的表达,分析它们与患者临床病理指标的相关性,初步探讨它们在子宫颈癌发生发展中的相互关系及作用机制。方法:经Trizol一步法提取40例子宫颈癌组织及30例正常子宫颈组织标本的总RNA,逆转录成cDNA,反转录RT-PCR法检测子宫颈癌组织及正常子宫颈组织中Survivin、NF-κB、STAT3 mRNA表达,分析Survivin、NF-κB、STAT3 mRNA表达及与临床病理指标之间的相关性。结果:子宫颈癌组织中Survivin、NF-κB、STAT3 mRNA表达水平显著高于正常子宫颈组织(P<0.05)。Ⅱb~Ⅲ a期子宫颈癌组织中三者mRNA的表达显著高于Ⅰ~Ⅱa期(P<0.01);子宫颈癌患者中有淋巴结转移者Survivin mRNA的表达明显高于无淋巴结转移者(P<0.01)。且STAT3表达与Survivin表达相关(r=0.483,P<0.05);Survivin表达与NF-κB表达相关(r=0.377, P<0.05)。结论:Survivin、NF-κB、STAT3 mRNA在子宫颈癌组织中表达上调,共同参与子宫颈癌的发生发展。

关键词: 宫颈癌, Survivin, NF-&kappa, B, STAT3

Abstract:

OBJECTIVE: To test expressions of Survivin,NF-κB and STAT3 in 40 cervical carcinoma tissue and 30 normal cervical tissue samples. The correlation between Survivin,NF-κB and STAT3 expression level and the clinical factors and pathology classification were analyzed,and the relationship and mechanism of Survivin,NF-κB and STAT3 in cervical carcinoma development were studied. METHODS:Trizol one step method was used to extract total RNA from both cervical carcinoma and normal tissues,mRNA reverse transcriptase cDNA,the polymerase chain reaction (RT-PCR half quantitative) method was used to measure Survivin,NF-κB and STAT3 mRNA expressions in cervical carcinoma and normal tissues. Further analysis of correlation between Survivin,NF-κB and STAT3 mRNA expression and the clinical pathological factors were performed. RESULTS:Survivin,NF-κB and STAT3 mRNA expressions in carcinoma tissue were significantly higher than the normal tissue (P<0.05). Survivin,NF-κB and STAT3 mRNA expression inⅡb-Ⅲa stage was significantly higher than Ⅰ-Ⅱa stage (P<0.01). The expression of Survivin mRNA in patients with lymph node metastasis was higher than those with no lymph node metastasis (P<0.01) . Survivin expression was related to STAT3 expression (r=0.483,P<0.05) and to NF-κB expression (r=0.377,P<0.05). CONCLUSION: Survivin,NF-κB and STAT3 expressions were upregulated in cervical carcinoma. All of these were closely related to the development and progression of cervical carcinoma.

Key words: cervical carcinoma, Survivin, NF-κB, STAT3