广东潮汕贲门癌组织中GST-π和P-gp的表达及其临床病理意义

doi:10.3969/j.issn.1004-616x.2016.01.008

癌变·畸变·突变 ›› 2016, Vol. 28 ›› Issue (1): 36-40.doi: 10.3969/j.issn.1004-616x.2016.01.008

广东潮汕贲门癌组织中GST-π和P-gp的表达及其临床病理意义

陈广灿¹, 洪良利², 黄杰雄², 谢澳斯¹, 郭丹³, 刘淑慧³

1. 汕头大学医学院第一附属医院胃肠外科, 广东汕头 515041;
2. 汕头大学医学院第一附属医院病理科, 广东汕头 515041;
3. 汕头大学医学院病理教研室, 广东汕头 515041

收稿日期:2015-09-01 修回日期:2015-12-21 出版日期:2016-01-31 发布日期:2016-01-31
通讯作者: 刘淑慧,E-mail:liushuhuistu@126.com E-mail:liushuhuistu@126.com
作者简介:陈广灿,E-mail:515776718@qq.com。
基金资助:
广东省自然科学基金资助(2014A030310139)

Expression of GST-π and P-gp in gastric cardia cancer and their clinical significance in the Chaoshan high risk area

CHEN Guangcan¹, HONG Liangli², HUANG Jiexiong², XIE Aosi¹, GUO Dan³, LIU Shuhui³

1. Department of Gastrointestinal Surgery, the First Affiliated Hospital of Shantou University Medical College, Shantou 515041;
2. Department of Pathology, the First Affiliated Hospital of Shantou University Medical College, Shantou 515041;
3. Department of Pathology, Shantou University Medical College, Shantou 515041, Guangdong, China

Received:2015-09-01 Revised:2015-12-21 Online:2016-01-31 Published:2016-01-31

摘要/Abstract

摘要： 目的: 探讨广东潮汕贲门癌患者癌组织中谷胱甘肽-巯基-转移酶-π(GST-π)和P-糖蛋白(P-gp)的表达情况及二者与临床病理指标的关系。方法: 采用免疫组织化学法检测87例贲门癌组织GST-π和P-gp的表达情况。根据表达情况,利用Youden index法绘制患者的年龄和肿瘤最长径ROC曲线,并将患者分成高表达组和低表达组比较其与临床病理指标的关系。结果: 贲门癌组织中GST-π和P-gp表达阳性率分别为85.05%和93.1%。根据ROC曲线的结果确定年龄和肿瘤最长径分组的界值。贲门癌GST-π表达与脉管是否浸润有关,有脉管浸润者高表达率明显高于无脉管浸润者(P<0.01);P-gp的表达与年龄和组织学类型相关,患者年龄增大表达率增高(P<0.05),黏液腺癌的高表达率明显高于非黏液腺癌(P<0.05)。贲门癌GST-π的表达与P-gp的表达呈正相关(r=0.828,P<0.01)。结论: GST-π和P-gp在潮汕贲门癌组织中阳性表达率高,两者的表达呈正相关。GST-π高表达与脉管浸润相关,P-gp高表达与高龄患者和贲门黏液腺癌相关。

关键词: 贲门癌, 谷胱甘肽-巯基-转移酶-π, P-糖蛋白, 免疫组织化学

Abstract: OBJECTIVE: To study the expression of glutathione-s-transferase-π (GST-π) and P-glycoprotein (P-gp) in gastric cardia the cancer in the Chaoshan high risk area and their relationships with clinical pathological characteristics of gastric cardia cancer. METHODS: Immunohistochemical staining was used to detect the expression of GST-π and P-gp in 87 cases of gastric cardia cancer. The correlations between GST-π,P-gp expression and the clinical characteristics of gastric cardia cancer were analyzed. ROC curves of age and maximum tumor diameter were plotted. RESULTS: The positive expression rate of GST-π and P-gp in gastric cardia cancer were 85.05% and 93.1%,respectively. The cutoff points of grouping for age and maximum tumor diameter depended upon the results of ROC curves. The expression of GST-π was related to lymph-vascular space invasion. The expression rate of GST-π of patients with lymph-vascular space invasion was signi?cantly higher than that without the invasion(P<0.05). Expression of P-gp was related to the age of patients and the histological type of gastric cardia cancer. Cancer tissues from elder patients and mucinous adenocarcinomas showed significantly higher rate of expression of P-gp (P<0.05). GST-π expression was positively correlated to P-gp expression (P<0.01). CONCLUSION: GST-π and P-gp expression were high in gastric cardia cancer in the Chaoshan area. Their expression was positively correlated. High GST-π expression was associated with lymph-vascular space invasion. High P-gp expression was associated with elder patients and mucinous adenocarcinoma tissues.

Key words: gastric cardia cancer, glutathione-s-transferase-π, P-glycoprotein, immunohistochemistry

中图分类号:

R730.2

陈广灿, 洪良利, 黄杰雄, 谢澳斯, 郭丹, 刘淑慧. 广东潮汕贲门癌组织中GST-π和P-gp的表达及其临床病理意义[J]. 癌变·畸变·突变, 2016, 28(1): 36-40.

CHEN Guangcan, HONG Liangli, HUANG Jiexiong, XIE Aosi, GUO Dan, LIU Shuhui. Expression of GST-π and P-gp in gastric cardia cancer and their clinical significance in the Chaoshan high risk area[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(1): 36-40.

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广东潮汕贲门癌组织中GST-π和P-gp的表达及其临床病理意义

Expression of GST-π and P-gp in gastric cardia cancer and their clinical significance in the Chaoshan high risk area

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