γ-聚谷氨酸对大鼠的致畸作用研究

doi:10.3969/j.issn.1004-616x.2026.01.009

摘要/Abstract

摘要： 目的：探讨γ-聚谷氨酸对大鼠的致畸作用。方法：按照《GB 15193.14-2015食品国家安全标准》，将80只受孕后SD大鼠分为阴性对照组、阳性(环磷酰胺)对照组和低、中、高剂量(分别为750、1 500、3 000 mg/kg)γ-聚谷氨酸组。SD大鼠于受孕后第6~15天每天按设计剂量分别掺入基础饲料中经口给予γ-聚谷氨酸，阳性对照组在受孕后的第12天一次性腹腔注射给予15 mg/kg的环磷酰胺。各组于妊娠第20天处死孕鼠，剖腹检查亲代受孕和胎鼠发育情况。取出子宫，称取子宫连胎质量，记录黄体数、死胎数、活胎数及着床数，并对胎鼠外观形态、内脏、骨骼畸形进行检查。结果： γ-聚谷氨酸各剂量组动物的子宫连胎质量、黄体数、着床数、吸收胎数、死胎数、活胎数以及胎鼠外观形态、内脏、骨骼畸形等各项指标与阴性对照组比较差异无统计学意义(均为P>0.05)，环磷酰胺组各项指标与阴性对照组比较差异均有统计学意义(P<0.05或P<0.01)。结论：在本试验条件下，γ-聚谷氨酸对孕鼠体质量、大鼠生殖能力及胎鼠生长发育无明显不良影响，未见γ-聚谷氨酸对胎鼠外观、骨骼和内脏有致畸作用。

关键词: γ-聚谷氨酸, 致畸, 生长发育, 大鼠

Abstract: OBJECTIVE： To investigate teratogenic effect of γ-polyglutamic acid in SD rats. METHODS： Pregnant rats (n=80) were divided into negative control group，positive (cyclophosphamide) control group，and low，medium，and high dose (750，1 500，3 000 mg/kg，respectively) γ-polyglutamic acid groups according to the National Food Safety Standard GB 15193.14-2015 and to their body weight in a zigzag pattern. Rats were orally administered with γ-polyglutamic acid at the designed dose per day from day 6 to day 15 after conception，while the positive control group was given a one-time intraperitoneal injection of 15 mg/kg cyclophosphamide on day 12 of conception. On the 20th day of pregnancy，they were euthanized，and their parental pregnancy and fetal development were examined by caesarean section. Uteri connected to fetuses were removed and weighed， the number of corpora lutea were recorded as well as premature deaths， late stillbirths，live births，and implantation. Fetel rats were inspected for the appearance，internal organs，and skeletal deformities. RESULTS： There was no statistically significant difference (P>0.05) in the uterine mass， corpus luteum number， implantation number， absorbed fetus， stillbirth， live fetus number， as well as the appearance，visceral，and skeletal deformities of the fetal mice between the γ-polyglutamic acid dose groups and the negative control group. However，there was a statistically significant difference (P<0.05 or P<0.01) in all indicators between the cyclophosphamide (15 mg/kg) group and the negative control group. CONCLUSION： Under the conditions of this experiment，γ-polyglutamic acid had no significant adverse effects on the body mass of pregnant rats，reproductive ability of rats，and growth and development of fetal rats. No teratogenic effects of γ-polyglutamic acid on the appearance，bones，and internal organs of fetal mice were observed.

Key words: γ-polyglutamic acid, teratogenicity, growth and development, rat

中图分类号:

陆罗定, 奚清丽, 张颖. γ-聚谷氨酸对大鼠的致畸作用研究[J]. 癌变·畸变·突变, 2026, 38(1): 54-58.

LU Luoding, XI Qingli, ZHANG Ying. Teratogenic effect of γ-polyglutamic acid in rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2026, 38(1): 54-58.

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