关键词: 乳腺癌, maspin基因, 甲基化, 亚硫酸氢钠测序法

Abstract: BACKGROUND AND AIM: To investigate the relationship between the inactivation of maspin gene and breast cancer. MATERIALS AND METHODS: The expression of Maspin protein in breast cancer tissue, adjacent non-tumor tissue, and distal normal tissue from 30 cases were detected by immunohistochemical method. The CpG islands methylation states of maspin promoter in breast cancer tissue were studied by bisulfite sequencing PCR (BSP). RESULTS: The protein expression levels of maspin in breast cancer were significantly lower than that in adjacent non-tumor tissue (P<0.05), and in distal normal tissue (P<0.01). The methylation incidence of maspin gene promoter CpG islands in breast cancer tissue was 98.72％. CONCLUSION: These findings suggest a close correlation between the inactivation of maspin gene and breast carcinogenesis. The aberrant methylation of promoter maybe is important mechanism of maspin gene inactivation in human breast carcinogenesis.

Key words: breast neoplasms;maspin gene, DNA methylation;bisulfite sequencing PCR