电离辐射对体外血管内皮细胞早衰的影响

doi:10.3969/j.issn.1004-616x.2025.01.003

摘要/Abstract

摘要： 目的：探讨人微血管内皮细胞(HMEC-1)在电离辐射照射后是否发生早衰及其产生的机制。方法：以剂量率11.79 mGy/s的X射线照射HMEC-1细胞，设置为0(对照组)、2和4 Gy剂量组，分别照射0、2.83和5.65 min后继续培养24、48和72 h。采用CCK-8试剂盒检测电离辐射暴露后细胞活力的变化；β-半乳糖苷酶染色试剂盒检测辐照后细胞衰老的发生情况；利用吉姆萨染色观察辐照后细胞形态变化；Western blot法检测衰老标志蛋白P53和P21的表达水平。结果：与对照组相比，4 Gy剂量组照射后 0 h，细胞活力显著下降(P<0.05)；2和4 Gy X射线照射后24、48和72 h的细胞活力均显著下降(P<0.01)；且2和4 Gy X射线照射后72 h的细胞β-半乳糖苷酶染色显著增加(P<0.05)。与未发生衰老细胞相比，发生早衰细胞的体积变大，随着照射剂量增加，细胞形态变化越明显；与对照组相比，照射组细胞衰老相关蛋白P53和P21的表达水平均显著升高(P<0.05)。结论：电离辐射可通过激活P53-P21通路引起HMEC-1细胞发生早衰。

关键词: 电离辐射, X射线, 血管内皮细胞, 早衰

Abstract: OBJECTIVE： To investigate whether premature aging occurs after irradiation of human microvascular endothelial cells (HMEC-1) with ionizing radiation and the mechanism of its generation. METHODS： HMEC-1 cells were irradiated with X-rays at a dose rate of 11.79 mGy/s for 0，2 and 4 Gy，for 0，2.83 and 5.65 min，respectively，and cultured for 24，48 and 72 h after irradiation. The CCK-8 kit was used to detect the changes in cell viability after exposure to ionizing radiation；the β-galactosidase staining kit was used to detect the onset of cellular senescence after irradiation；Giemsa staining was used to observe the changes in the morphology of irradiated cells；and the expression levels of the senescence marker proteins，P53 and P21，were detected using the Western blot method. RESULTS： Compared with the unirradiated group，cell viability decreased significantly at 0 h after irradiation in the 4 Gy dose group (P<0.05)；cell viability decreased significantly at 24，48 and 72 h after irradiation in the 2 and 4 Gy X-ray irradiation groups (P<0.01)；and β-galactosidase staining of the cells at 72 h after irradiation in the 2 and 4 Gy X-rays irradiation groups increased significantly compared with the unirradiated group (P<0.05). And compared with the non-senescent cells，the morphology of the cells that underwent premature senescence became larger，and with the increase of irradiation dose，the more obvious the changes in cell morphology；compared with the non-irradiated group，the expression level of cellular senescence-related proteins P53 and P21 in the irradiated group was significantly elevated (P<0.05). CONCLUSION： Ionizing radiation can cause premature senescence of HMEC-1 cells by activating the P53-P21 pathway.

Key words: ionising radiation, X-ray, vascular endothelial cells, premature aging

中图分类号:

Q691.5

伊如罕, 陆雪, 蔡恬静, 高玲. 电离辐射对体外血管内皮细胞早衰的影响[J]. 癌变·畸变·突变, 2025, 37(1): 16-20.

YI Ruhan, LU Xue, CAI Tianjing, GAO Ling. The effect of ionizing radiation on premature death of vascular endothelial cells in vitro[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2025, 37(1): 16-20.

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