癌变·畸变·突变 ›› 2002, Vol. 14 ›› Issue (4): 210-213.doi: 10.3969/j.issn.1004-616x.2002.04.002

• 论著 • 上一篇    下一篇

吡虫啉和抑食肼对人体外周血淋巴细胞遗传物质的影响

封少龙1, 孔志明2, 王五香3, 王新明1, 彭平安1   

  1. 1. 有机地球化学国家重点实验室, 中国科学院广州地球化学研究所有机室, 广东　广州　510640 ; 　2. 南京大学环境学院,污染控制与资源化国家重点实验室,江苏　南京　210093 ; 　3. 湖南省衡南县教师进修学校,湖南　衡阳　421141)
  • 收稿日期:2002-03-12 修回日期:2002-05-29 出版日期:2002-10-30 发布日期:2002-10-30
  • 通讯作者: 封少龙

EFFECT OF IMIDACLOPRID AND RH25849 ON GENETIC MATERIAL OF HUMAN PERIPHERAL LYMPHOCYTES

FENG Shao-long1, KONG Zhi-ming2, WANG Wu-xiang3, WANG Xing-ming1, PENG Ping-an1   

  1. 1. The State Key Laboratory of Organic Geochemistry, Chinese Academy of Sciences Guangzhou Institue of Geochemistry, Guangzhou 510640, China; 2. The State Key Laboratory of Pollution Control and Resource Reuse, the School of Environment, Nanjing University, Nanjing 210093, China; 3. The Refresher School of teacher in Hengnan Country, Hengyang 421141, China
  • Received:2002-03-12 Revised:2002-05-29 Online:2002-10-30 Published:2002-10-30

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摘要： 目的: 研究两种新型杀虫剂- 吡虫啉和抑食肼对人体外周血淋巴细胞遗传物质的影响。方法:人体外周血淋巴细胞的微核试验,姐妹染色单体互换试验(SCE) 以及单细胞凝胶电泳试验(SCGE ,又名彗星试验) 。结果:在低浓度(吡虫啉为0. 05mg/ L ,抑食肼为5 mg/ L) 时,它们对微核和SCE 的影响与对照组相比无显著性差异( P > 0. 05) ,当浓度升高(吡虫啉为0. 1mg/ L ,抑食肼为25 mg/ L) 时,则有显著性差异( P < 0. 05) 。而彗星试验在各试验组与对照组相比都有极显著性差异( P <0. 01) ,且存在明显的剂量- 效应关系( r = 0. 995 , r = 0. 965) 。结论:吡虫啉和抑食肼对人外周血淋巴细胞的遗传物质都具有一定程度的损伤作用,相比之下,吡虫啉比抑食肼具有更大的毒性。

关键词: 微核, 姐妹染色单体互换(SCE), 彗星试验, 吡虫啉, 抑食肼

Abstract: Purpose : To study the effect s of the two new-type pesticides , imidacloprid and RH25849 , on the DNA of human peripheral lymphocytes. Methods : Micronuclei test , SCE test and comet assay were employed in the experiment . Results : At the low dose (imidacloprid 0. 05 mg/ L and RH25849 5 mg/ L) , there were no effect on the micronuclei rate and the SCE rate , compared with the negative cont rolled groups ( P > 0. 05) . But there were adverse effect s when the dose increased , and the difference was significant as compared with the negative cont rol groups ( P < 0. 05) . In the comet assay , starting f rom the lowest dose the two pesticides caused DNA damages in the human peripheral lymphocytes as compared with the cont rol groups , and the differences were significant ( P < 0. 01) , and there were dose dependent relationship ( r = 0. 995 , r = 0. 965) . ConclusionBoth imidacloprid and RH-5849 damaged the DNA of human peripheral lymphocytes. Comparatively. Imidacloprid was more toxic than RH25849.

Key words: micronuclei, SCE, comet test, imidacloprid : RH-5849

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