癌变·畸变·突变 ›› 2002, Vol. 14 ›› Issue (4): 234-237.doi: 10.3969/j.issn.1004-616x.2002.04.009

• 检测研究 • 上一篇    下一篇

子宫营养试验检测双酚A 的雌激素样活性

黄毅娜, 程薇波, 徐培渝, 余文三, 王文东, 王正书, 庞定国, 刘玉清   

  1. 四川大学华西公共卫生学院, 四川　成都　610041)
  • 收稿日期:2002-06-06 修回日期:2002-07-10 出版日期:2002-10-30 发布日期:2002-10-30
  • 通讯作者: 黄毅娜

IMMATURE SD RAT UTEROTROPHIC ASSAY OF BISPHENOL A

HUANG Yi-na, CHENG Wei-bo, XU Pei-yu, YU Wen-san, etal.   

  1. School of Public Health, Si Chuan University, Chengdu 610041, China
  • Received:2002-06-06 Revised:2002-07-10 Online:2002-10-30 Published:2002-10-30

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摘要： 目的: 采用子宫营养试验,检测双酚A(BisphenolA ,BPA) 的雌激素样活性,初步探讨其作用机制。方法:未成年SD 雌性大鼠(21d) 100 只,分为BPA 200 mg/ kg、400 mg/ kg 和800 mg/ kg 3 个剂量组,溶剂对照组(花生油) 和阳性对照组(苯甲酸雌二,Estradiolnzoate ,E2B) 0. 4 mg/ kg ,每天一次灌胃给药,连续给予3 d 和7 d。于末次给药的第24 h 处死动物取子宫。结果:3 d 和7 d 两个时段,E2B、BPA 400 mg/ kg 和800 mg/ kg 2 个剂量组子宫湿重、子宫/ 体重比、平滑肌厚度和宫腔上皮高度与溶剂对照组相比有显著性差异( P < 0. 01) ,有明显的剂量效应关系。给予BPA 7 d 还使未成年SD 雌性大鼠阴道开口时间提前。结论:BPA 具有弱雌激素样效应,子宫湿重增加可能与平滑肌增生有关。

关键词: 子宫营养试验, 双酚A, 雌激素样活性, 幼雌SD 大鼠

Abstract: Purpose : Uterot rophic assay has been kown efficient for detecting endocrine disrupters. To examine the est rogenic activity of bisphenol A(BPA) using the immature female SD rat . Methods : The vehicle cont rol(the peanut oil) , positive cont rol (est radiol benzoate , E2B , 0. 4 mg/ kg ) and BPA(200 mg/ kg ,400 mg/ kg and 800 mg/ kg) were given orally for 3 and 7 consecutive days beginning postnatal day 21. Then rat s were sacrificed 24 hour after the last administ ration. Results : Uterine weight , uterine/ weight , the thickness of the myomet rium and the height of the luminal epithelium increased in E2B , BPA 400 mg/ kg and 800 mg/ kg group ( P <0. 01) with a dose2response relationship. Administered BPA for 7 days , the vaginal opening time of the immature female SD rat s advanced. Conclusion : BPA has weak est rogenic activity in SD rat . The hypert rophy andhyperplasia of myomet rial cell of uterus may induce the uterine weight increase.

Key words: uterot rophic assay, bisphenol A, est rogenic activity, immature female SD rat

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