癌变·畸变·突变 ›› 2003, Vol. 15 ›› Issue (3): 157-160.doi: 10.3969/j.issn.1004-616x.2003.03.009

• 检测研究 • 上一篇    下一篇

杂多化合物九钨三钛硅酸盐的急性毒性和致突变性研究

梅树江1，刘　娅2，王宝贵2，章培标3，张桂英2，赵林伊2   

  1. 1.汕头大学医学院生物教研室,　广东　汕头　515031；2.吉林大学公共卫生学院,　吉林　长春　130021；3.长春市卫生局卫生监督所，吉林　长春　130021
  • 收稿日期:2003-04-20 修回日期:2003-05-15 出版日期:2003-07-30 发布日期:2003-07-30
  • 通讯作者: 梅树江

STUDY ON ACUTE TOXICITY AND MUTAGENTICITY OF POLYOXOANIONS α-K 8H 2 [SiW 9Ti 3O 40]•15H 2O

MEI Shu-jiang,LIU Ya,WANG Bao- Gui，et al   

  1. 1.Department of biiology,Shantou University Medical College,Shantou 515031,China;2.College o f Preventive Medicine,Jilin University,Changchun 130021,China;3.Health Supervision Office of Changchun Health Bureau Changchun 130021，China
  • Received:2003-04-20 Revised:2003-05-15 Online:2003-07-30 Published:2003-07-30
  • Contact: MEI Shu-jiang

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摘要： 目的： 对杂多化合物九钨三钛硅酸盐(α-K8H2[SiW9Ti3O40]•15H2O，简称α-Ti3)进行急性毒性和致突变性研究，为临床应用提供科学依据。方法： 采用小鼠经口急性毒性试验、Ames试验、小鼠骨髓嗜多染红细胞微核试验及CHO细胞染色体畸变试验等方法进行检测。结果： ①小鼠经口急性毒性试验，LD50为2 055.36 mg/kg，属低毒物质；②α-Ti3浓度在31.3～500 μg /皿范围内，无论加S 9与否，每皿菌落回变数均未超过阴性对照组的两倍；③微核试验在25、50、125 mg/kg 3个剂量水平，各剂量组对小鼠骨髓嗜多染红细胞微核千分率均未见明显增加，与阴性对照组比较无显著性差异(P>0.05)；④α-Ti3浓度在40～320 μg/ml范围内，无论加S 9与否，CHO染色体畸变率与阴性对照组比较无显著性差异(P>0.05)。结论： 杂多化合物九钨三钛硅酸盐毒性较低，在本试验条件下，诱变性试验结果为阴性。

关键词: 杂多化合物, 九钨三钛硅酸盐, LD50, Ames试验, 微核, 染色体畸变

Abstract: Purpose: To study the oral acute toxicity and mutagenicity of Polyoxometalates α-K8H2[SiW9Ti3O40]•15H2O for clinic application(α-Ti3). Methods: The studies were conducted with acute toxicity test,Ames test,micronuclei test and chromosome aberration test on CHO cell in vitro. Results: ① LD50 in mice was 2 055.36 mg/kg ,it was a low-grade toxicity compound .②The results of Ames test showed no mutagenic effects with the concentration ranged from 31.3 to 500 μg/plate with or without S9 mixture added. ③In micronuclei test,the mice orally received 25,50,125 mg/kg level of α-Ti3,no mutagenic effects were observed,and no significant increase was observed on polychromatophil.④The chromosome aberration ratio of α-Ti3 was no significant discrepancy compared with the control on CHO cells,with the concentration ranged from 40 to 320 μg /ml with or without S 9 mixture added. Conclusion： The toxicity of α-Ti 3 was low and no mutagenicity was observed in the experiment.

Key words: Polyoxometalates, α-K8 H2 [SiW9Ti3 O 40]•, 15H2 O, LD50, Ames test, micronuclei, chromosome aberration

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